Fine genetic mapping fails to dissociate durable stem rust resistance gene Sr2 from pseudo-black chaff in common wheat (Triticum aestivum L.) |
| |
Authors: | R Kota W Spielmeyer R A McIntosh E S Lagudah |
| |
Institution: | (1) Graingene, CSIRO Plant Industry, GPO Box 1600, Canberra, 2601, ACT, Australia;(2) CSIRO Plant Industry, GPO Box 1600, Canberra, 2601, ACT, Australia;(3) Plant Breeding Institute, University of Sydney, PMB 11, Camden, 2570, NSW, Australia |
| |
Abstract: | The broad-spectrum stem rust resistance gene Sr2 has provided protection in wheat against Puccinia graminis Pers. f. sp. tritici for over 80 years. The Sr2 gene and an associated dark pigmentation trait, pseudo-black chaff (PBC), have previously been localized to the short arm
of chromosome 3B. In a first step towards the positional-based cloning of Sr2, we constructed a high-resolution map of this region. The wheat EST (wEST) deletion bin mapping project provided tightly
linked cDNA markers. The rice genome sequence was used to infer the putative gene order for orthologous wheat genes and provide
additional markers once the syntenic interval in rice was identified. We used this approach to map six wESTs that were collinear
with the physical order of the corresponding genes on rice chromosome 1 suggesting there are no major re-arrangements between
wheat and rice in this region. We were unable to separate by recombination the tightly linked morphological trait, PBC from
the stem rust resistance gene suggesting that either a single gene or two tightly linked genes control both traits. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|