Development of a microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L.) |
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Authors: | X Chen S Temnykh Y Xu Y G Cho S R McCouch |
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Institution: | (1) Department of Plant Breeding and Biometry, 252 Emerson Hall, Cornell University, Ithaca, NY 14853-1901, USA, US;(2) National Institute of Agricultural Science and Technology, Suweon, Korea, KR |
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Abstract: | Ninety-four newly developed microsatellite markers were integrated into existing RFLP framework maps of four rice populations,
including two doubled haploid, a recombinant inbred, and an interspecific backcross population. These simple sequence repeats
(SSR) were predominantly poly(GA) motifs, targetted because of their abundance in rice. They were isolated from a previously
described sheared library and a newly constructed enzyme-digested library. Differences in the average length of poly(GA) tracts
were observed for clones isolated from the two libraries. The length of GA motifs averaged 21 repeat units for clones isolated
from the Tsp-509-digested library, while motifs averaged 17 units for clones from the sheared library. There was no evidence of clustering
of microsatellite markers near centromeres or telomeres. Mapping of the 94 newly developed markers as well as of 27 previously
reported microsatellites provided genome-wide coverage of the 12 chromosomes, with an average distance of 1 SSLP (simple sequence
repeat polymorphism) per 16–20 cM.
Received: 13 February 1997/Accepted: 28 February 1997 |
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Keywords: | Microsatellite Simple sequence repeats (SSR) Simple sequence length polymorphism(SSLP) Linkage map Molecular map Rice (Oryza sativa) |
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