Differentiation and classification of parental lines and favorable genic interactions affecting F1 fertility in distant crosses of rice (Oryza sativa L.) |
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| Authors: | R H Li C G Xue L P Yuan Y Q He C Q Sun S B Yu X H Li X K Wang |
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| Institution: | (1) Department of Plant Genetics and Breeding, China Agricultural University, Haidian, Beijing 10094, China Fax: 086-010-62891055 E-mail: rhlksy@ihw.com.cn, CN;(2) State Key Laboratory in Genetic Improvements of Crops, Huazhong Agricultural University, Wuhan 430070, China, CN;(3) National Hybrid Rice Research and Development Center, Changsha 410125, China, CN |
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| Abstract: | This study was intended to investigate the extent of genetic differentiation in parental lines of rice hybrids and to analyze
the genetic basis underlying the fertility phenomenon in distant crosses. Two subsets of rice material (111 entries in total)
were used, including 81 doubled-haploid (DH) lines and 30 Indica and Japonica rice varieties or lines (as a control). The
DH lines was derived from a heterotic Indica/Japonica cross (Gui630/02428) by anther culture. The materials in the control
represent a broad spectrum of the Asian cultivated rice gene pool including landraces, primitive cultivars, historically important
cultivars, modern elite cultivars, super rice and parents of superior hybrids. In accordance with the NC II design, 57 out
of the DH lines were test-crossed to two important wide compatibility lines: photoperiod-sensitive genetic male sterile (PGMS)
line N422s and thermo-sensitive genetic male sterile (TGMS) line Peiai64s. The F1s and their parents, 182 entries in total, were examined for the performance of seven traits in a replicated field trial.
All the rice materials was surveyed for polymorphisms using 92 RFLP markers selected from two published molecular marker linkage
maps. Genotypes of the F1 hybrids at the molecular-marker loci were deduced from the parental genotypes. The analysis showed that there were two types
of genetic differentiation in the two subsets of rice material; that is, qualitative differentiation in the control and quantitative
differentiation in the DH lines. In addition, favorable genic interactions (both intra- or inter-locus) contributed to better
increase the fertility in hybrids of distant crosses through incorporation of a wide-compatibility line as the female parent.
Favorable genic interactions can be applied in hybrid rice breeding programs by selecting parents with an appropriate extent
of genetic differentiation.
Received: 5 June 1997 / Accepted: 10 September 1997 |
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| Keywords: | Oryza sativa L Genetic differentiation Classification of parental lines Restriction fragment length polymorphism (RFLP) Genic interaction |
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