Fast conventional Fmoc solid-phase peptide synthesis with HCTU. |
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Authors: | Christina A Hood German Fuentes Hirendra Patel Karen Page Mahendra Menakuru Jae H Park |
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Institution: | Department of Chemistry, Protein Technologies Inc., Tucson, Arizona, 4675 South Coach Drive, Tucson, Arizona 85714, USA. |
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Abstract: | 1H-Benzotriazolium 1-bis(dimethyl-amino)methylene]-5-chloro-hexafluorophosphate (1-),3-oxide (HCTU) is a nontoxic, nonirritating and noncorrosive coupling reagent. Seven biologically active peptides (GHRP-6, (65-74)ACP, oxytocin, G-LHRH, C-peptide, hAmylin(1-37), and beta-amyloid(1-42)) were synthesized with reaction times reduced to deprotection times of 3 min or less and coupling times of 5 min or less using HCTU as the coupling reagent. Expensive coupling reagents or special techniques were not used. Total peptide synthesis times were dramatically reduced by as much as 42.5 h (1.8 days) without reducing the crude peptide purities. It was shown that HCTU can be used as an affordable, efficient coupling reagent for fast Fmoc solid-phase peptide synthesis. |
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Keywords: | HCTU fast Fmoc SPPS human amylin (1–37) oxytocin β‐amyloid |
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