General method for selective labelling of double‐chain cysteine‐rich peptides with a lanthanide chelate via solid‐phase synthesis |
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| Authors: | Fazel Shabanpoor Frances Separovic John D Wade |
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| Institution: | 1. Howard Florey Institute, University of Melbourne, Victoria 3010, Australia;2. School of Chemistry, University of Melbourne, Melbourne, Victoria 3010, Australia |
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| Abstract: | The use of lanthanides in preference to radioisotopes as probes for various biological assays has gained enormous popularity. The introduction of lanthanide chelates to peptides/proteins can be carried out either in solution using a commercially available labelling kit or by solid‐phase peptide synthesis using an appropriate lanthanide chelate. Herein, a detailed protocol for the latter is provided for the labelling of peptides or small proteins with diethylenetriamine‐N, N, N″, N″‐tetra‐tert‐butyl acetate‐N′‐acetic acid (DTPA) chelate or other similar chelates on a solid support using a chimeric insulin‐like peptide composed of human insulin‐like peptide 5 (INSL5) A‐chain and relaxin‐3 B‐chain as a model peptide. Copyright © 2011 European Peptide Society and John Wiley & Sons, Ltd. |
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| Keywords: | lanthanide chelate time‐resolved fluorescent solid phase |
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