Visualization of ribosomal DNA loci in spore interphasic nuclei of glomalean fungi by fluorescence in situ hybridization |
| |
| Authors: | Sophie Trouvelot Diederik van Tuinen Mohamed Hijri V Gianinazzi-Pearson |
| |
| Institution: | (1) Laboratoire de Phytoparasitologie INRA/CNRS, Centre de Microbiologie du Sol et de l'Environnement, INRA, BV 1540, F-21034 Dijon Cedex, France e-mail : gianina@epoisses.inra.fr, Fax: +333-80-63-32-63, FR |
| |
| Abstract: | Fluorescence in situ hybridization (FISH) was applied to interphasic nuclei isolated from spores of four species of AM fungi
: Scutellospora castanea, Glomus mosseae, Glomus intraradices and Gigaspora rosea. Ribosomal DNA loci were visualized using digoxigenin-labeled 25 S rDNA probes obtained by nested PCR. Several hybridization
sites were detected per nucleus and an internuclear variability was observed in the number of loci. This is the first report
of successful application of FISH to analyse the genomes of glomalean fungi.
Accepted: 16 September 1998 |
| |
| Keywords: | Arbuscular mycorrhiza Spore nuclei Fluorescence in situ hybridization Ribosomal DNA loci |
| 本文献已被 SpringerLink 等数据库收录！ |
