Community structure of arbuscular mycorrhizal fungi in a primary successional volcanic desert on the southeast slope of Mount Fuji |
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Authors: | Bingyun Wu Taizo Hogetsu Katsunori Isobe Ryuichi Ishii |
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Institution: | (1) Department of Forest Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1 Bunkyo-ku, Tokyo 113-8657, Japan;(2) Department of Plant Science and Resources, College of Bioresource Sciences, Nihon University, Kameino 1866, Fujisawa-shi 252-8510, Kanagawa, Japan |
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Abstract: | Community structure of arbuscular mycorrhizal fungi (AMF), evaluated as spore samples and mycorrhizal roots of four herbaceous
plant species, was investigated at different altitudes in a primary successional volcanic desert on Mount Fuji using molecular
methods (fragment and sequence analysis of the large ribosomal subunit RNA gene). In total, 17 different AMF clades were identified,
and most were members of the Glomaceae, Acaulosporaceae, and Gigasporaceae. The AMF community structures detected by spore
sampling were inconsistent with those from plant roots. Of all AMF clades, six (35.3%) were detected only on the basis of
spores, six (35.3%) only in roots, and five corresponded to both spores and roots (29.4%). Although an Acaulospora species was the most dominant among spores (67.1%), it accounted for only 6.8% in root samples. A species analysis of AMF
communities at different altitudes demonstrated that AMF species diversity increased as altitude decreased and that the species
enrichment at lower altitudes resulted from the addition of new species rather than species replacement. The inconsistencies
in the species composition of spore communities with those in roots and the change in species diversity with altitude are
discussed. |
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Keywords: | Altitude AMF Diversity Large ribosomal subunit RNA gene (LSU rDNA) Primary succession Terminal restriction fragment-length polymorphism (t-RFLP) |
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