Cell-free Studies on the Regulation of the Arabinose Operon |
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| Authors: | GEOFFREY ZUBAY LINDA GIELOW ELLIS ENGLESBERG |
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| Institution: | 1.Department of Biological Sciences,Columbia University,;2.Department of Biological Sciences,University of California,Santa |
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| Abstract: | A DNA directed, cell-free system which synthesized L-ribulokinase coded by the L-arabinose operon ara OIBAD, has been developed. L-arabinose but not the araC gene product is required for the expression of this operon and, in addition, cyclic AMP and guanosine tetraphosphate are needed for optimal synthesis. |
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