| 百合病毒的DNA芯片检测技术研究 |
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| 引用本文： | 王进忠,贾慧,文思远,王升启,张民照,赵祥云,董金皋.百合病毒的DNA芯片检测技术研究[J].Virologica Sinica,2005,20(4):429-433. |
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| 作者姓名： | 王进忠 贾慧 文思远 王升启 张民照 赵祥云 董金皋 |
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| 作者单位： | 北京农学院植物科学技术系，北京农学院植物科学技术系，军事医学科学院放射医学研究所，军事医学科学院放射医学研究所，北京农学院植物科学技术系，北京市农业应用新技术重点实验室，北农业大学生命科学学院 北京102206，北京市农业应用新技术重点实验室，北京102206，北京102206，北京1000850，北京1000850，北京102206，北京102206，河北保定071001 |
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| 基金项目： | 北京市自然科学基金(5043026) |
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| 摘 要： | 根据已知的黄瓜花叶病毒,百合无症病毒、百合斑驳病毒基因核苷酸序列,设计引物和探针,制备寡核苷酸芯片。用Cy3标记核苷酸引物,不对称RT-PCR扩增产物与芯片上的寡核苷酸探针杂交,荧光扫描仪检测并分析信号。研究制备的基因芯片能够检测侵染百合的3种重要病毒核酸的特异性荧光信号,该项技术具有特异、灵敏、快速的优点。
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| 关 键 词： | 黄瓜花叶病毒 百合无症病毒 百合斑驳病毒 基因芯片 检测 |
| 收稿时间： | 2005-01-31 |
Detection for CMV, LSV, LMoV Infected Lily with DNA Microarry Techniques |
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| Wang JinZhong;Gu Hui;Wen SaiYuan;Wang ShengQi;Zhang MinZhao;Zhao XiangYun;Dong JinGao.Detection for CMV, LSV, LMoV Infected Lily with DNA Microarry Techniques[J].中国病毒学(英文版),2005,20(4):429-433. |
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| Authors: | Wang JinZhong;Gu Hui;Wen SaiYuan;Wang ShengQi;Zhang MinZhao;Zhao XiangYun;Dong JinGao |
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| Abstract: | Specific primers and probes were designed according to conservative and specific DNA sequence of CMV,LSV and LMoV.The probes were spotted on glass slides to form microarrays The Cy3-labled single strand DNA fragments prepared by dissymmetical RT-PCR were hybridized with the probes on the glass slides .The microarrays were scanned and analyzed with a scanner. DNA microarray could detect different typed DNA of CMV,LSV and LMoV with adequate specificity and sensitivity . Microarray techniques establish a rapid,sensitive and specific diagnostic assay for lily viruses. The successfully developed DNA microarray and techniques might be a very useful method for diagnosis and prevention ,and widely applied in specific pathogen detection of diseases such as lily virus. |
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| Keywords: | Cucumber mosaic virus(CMV) Lily symptomless virus(LSV) Lily mottle virus(LMoV) DNA microarry Detection |
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