| 猪细小病毒VP2蛋白主要抗原表位基因真核表达载体的构建及表达 |
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| 引用本文： | 任雪枫,胡志华,谈国蕾,周斌,徐学清,郑其升,张晓勇,陈德胜,陈溥言.猪细小病毒VP2蛋白主要抗原表位基因真核表达载体的构建及表达[J].Virologica Sinica,2004,19(6):636-638. |
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| 作者姓名： | 任雪枫 胡志华 谈国蕾 周斌 徐学清 郑其升 张晓勇 陈德胜 陈溥言 |
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| 作者单位： | 南京农业大学动物医学院农业部动物疫病诊断与免疫重点开放实验室 江苏南京210095
(任雪枫，胡志华，谈国蕾，周斌，徐学清，郑其升，张晓勇，陈德胜)，南京农业大学动物医学院农业部动物疫病诊断与免疫重点开放实验室 江苏南京210095(陈溥言) |
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| 基金项目： | 国家“863”高技术发展计划资助项目(2001AA249012)。 |
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| 摘 要： | Using a pair of specific primers designed according to the relevant nucleotide sequences from GenBank, the main antigen domain for VP2 gene of Porcine parvovirus was ampilified with PCR method using the genomic DNA as template. The PCR product was cloned into the expression vector pIREShyg to get a recombinant eukaryotic expression plasmid pIREShyg-VP2, which was then transfected into the CHO-K1 cells. The expressed product was detected by IFA after the positive cell clone was selected with hygromycin. The result revealed that the main antigen domain for VP2 gene of porcine parvovirus was stably expressed in CHO-K1 cells.
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| 关 键 词： | 猪细小病毒 VP2蛋白 潮霉素 CHO-K1细胞 |
Expression of the Domain Gene of Porcine Parvovirus VP2 with the Eukaryotic Vector pIREShyg |
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| REN Xue-feng,HU Zhi-hua,TAN Guo-lei,ZHOU Bing,XU Xue-qin,ZHENG Qi-sheng,Zhang Xiao-yong,CHEN De-sheng,CHEN Pu-yan.Expression of the Domain Gene of Porcine Parvovirus VP2 with the Eukaryotic Vector pIREShyg[J].中国病毒学(英文版),2004,19(6):636-638. |
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| Authors: | REN Xue-feng HU Zhi-hua TAN Guo-lei ZHOU Bing XU Xue-qin ZHENG Qi-sheng Zhang Xiao-yong CHEN De-sheng CHEN Pu-yan |
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| Institution: | REN Xue-feng,HU Zhi-hua,TAN Guo-lei,ZHOU Bing,XU Xue-qin,ZHENG Qi-sheng,Zhang Xiao-yong,CHEN De-sheng,CHEN Pu-yan** |
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| Abstract: | Using a pair of specific primers designed according to the relevant nucleotide sequences from GenBank, the main antigen domain for VP2 gene of Porcine parvovirus was ampilified with PCR method using the genomic DNA as template. The PCR product was cloned into the expression vector pIREShyg to get a recombinant eukaryotic expression plasmid pIREShyg-VP2, which was then transfected into the CHO-K1 cells. The expressed product was detected by IFA after the positive cell clone was selected with hygromycin. The result revealed that the main antigen domain for VP2 gene of porcine parvovirus was stably expressed in CHO-K1 cells. |
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| Keywords: | Porcine parvovirus (PPV) VP2 hygromycin CHO-K1 cells |
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