| 肾综合征出血热病毒在恙螨体内增殖的动态观察 |
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| 引用本文： | 张云,张家驹.肾综合征出血热病毒在恙螨体内增殖的动态观察[J].Virologica Sinica,1997,12(2):132-136. |
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| 作者姓名： | 张云 张家驹 |
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| 作者单位： | 南京军区军事医学研究所!南京，210002，南京军区军事医学研究所!南京，210002，南京军区军事医学研究所!南京，210002，陕西省卫生防疫站!西安，710054，陕西省卫生防疫站!西安，710054 |
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| 基金项目： | 国家自然科学基金!39470631 |
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| 摘 要： | 取饲养的小盾纤恙螨幼虫和若虫．每20d为一批制成无菌滤液接种Vero-E6细胞测定HFRSV滴度，动态观察HFRSV在螨体内的增殖情况，并用PCR技术检测恙螨幼虫和若虫体内HFRSV－RNA。结果如下：小盾纤恙螨幼虫期除60d一批外，其余各批均在不同时间内测出HFRSV滴度，3批若虫中亦有2批测出HFRSV滴度并检测到HFRSV－RNA。结果为小盾纤恙螨作为HFRS的传播媒介提供了进一步的证据。
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| 关 键 词： | 小盾纤恙螨 肾综合征出血热病毒 增殖 |
Dynamic Observation on Proliferation of HFRSV in Chigger Mite |
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| Zhang Yun ,Li Xianfu,Wu Guanghua.Dynamic Observation on Proliferation of HFRSV in Chigger Mite[J].中国病毒学(英文版),1997,12(2):132-136. |
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| Authors: | Zhang Yun Li Xianfu Wu Guanghua |
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| Abstract: | In order to observe further dynamic changes on proliferation of HFRSV in Leptotrombidium Scutellare, the raised Leptotrombidium scutellare were ground and sterilized with an interval of 20-40 days, the filterate of each batch was inoculated separately into Vero - E6 cells, titres of HFRSV and HFRSV-RNA were detected. The results confirmed that HFRSV were positive in most batches of larva. The above results indicated that the Leptotrombidlum sutellare is a transmitting vector of HFRSV. |
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| Keywords: | Leptotrombidium Sutellare Hemorrhagic Fever with Renal Syndrome Virus Pro-liferation |
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