| 高效价甘薯羽状斑驳病毒抗血清的制备 |
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| 引用本文： | 孟清,宋伯符.高效价甘薯羽状斑驳病毒抗血清的制备[J].Virologica Sinica,1994,9(2):151-156. |
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| 作者姓名： | 孟清 宋伯符 |
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| 作者单位： | 内蒙古大学生物系，国际马铃薯中心驻京办事处，徐州甘薯研究中心 |
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| 基金项目： | 国际马铃薯中心和内蒙古自然科学基金 |
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| 摘 要： | 用嫁接方法将甘薯羽状斑驳病毒（ＳＰＦＭＶ）接种到Ｉ．ｓｅｔｏｓａ上扩繁，以０．２ｍｏｌ／ＬｐＨ７．２ＰＢＫ缓冲液、垫层差速离心、蔗糖密度梯度离心提取纯化ＳＰＦＭＶ。纯化的ＳＰＦＭＶＯＤ２６０／２８０的比值为１．２５。将纯化的ＳＰＦＭＶ免疫家兔制备抗血清，在环状沉淀和微量沉淀试验中，用提纯病毒测定抗血清的效价均为１：４０９６；以ＳＰＦＭＶ－ＩｇＧ为第一抗体，应用Ｄｏｔ－ＥＬＩＳＡ对甘薯和Ｉ．ｓｅｌｏｓａ叶片中的ＳＰＦＭＶ分别作了测定。
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| 关 键 词： | 甘薯羽状斑驳病毒，病毒纯化，抗血清制备 |
Preparation of High Titer Antiserum to Sweet Potato Feathery Mottle Virus(SPFMV) |
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| Meng Qing,Zhang Heling,Song Bofu,Pang Ruijie,Xing Jiying,Yong Yunjia.Preparation of High Titer Antiserum to Sweet Potato Feathery Mottle Virus(SPFMV)[J].中国病毒学(英文版),1994,9(2):151-156. |
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| Authors: | Meng Qing Zhang Heling Song Bofu Pang Ruijie Xing Jiying Yong Yunjia |
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| Abstract: | weet potato feathery mottle virus(SPFMV)was inoculated to I.selosa to propagare by graftingand was purified wirh 0.2mol/L pH7.2 PBK.Further purification of SPFMV was conducted by sucrose cushion and sucrose density gradient centrifugation.Purified SPFMV had a OD260/280 ratio of1.25.Rilbbit was immuned with purifled viruses to prepare antiSerum.Antisera produced to purifiedvirus had a titer of 1:1096 in microprecipitin and ring precipitin tests.SPFMV in leaves of sweet potato and I.selosa were detected respectively by indirect enzyme-linked immunosorbent assay(ELISA)onnitreeellulose membranes. |
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| Keywords: | Sweet potato feathery mottle virus Purification of virus Preparation of antiserum |
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