鹦鹉源I型禽副粘病毒F和HN基因序列测定与分析 |
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引用本文: | 赵文华 宋建领 朱建波 信爱国 王金萍 本士/f&·rJ心■一 张念祖.鹦鹉源I型禽副粘病毒F和HN基因序列测定与分析[J].Virologica Sinica,2003,18(4):394-396. |
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作者姓名: | 赵文华 宋建领 朱建波 信爱国 王金萍 本士/f&·rJ心■一 张念祖 |
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摘 要: | Virion RNA was abstracted from purified type I Avian paramyxovirus strain YN-PA01(isolated from parrot)and used as a template. The fragment containing the fusion gene(F) and hemagglutinin-neuraminidase gene(HN) of the isolate was amplified by RT-PCR and cloned to the pMD18-T Vector. Using primer walking method the complete sequence of F-HN genes was obtained finally.And the respective amino acid sequence was deduced. Through relative software the phylogenetic trees on F gene and HN gene were constructed between strain YN-PA01 and reference strains. The results showed that strain YN-PA01 comparing with reference strains displays 98.7%-83.2% nucleotide homology and 98.1%-86.2% amino acid homology on F gene; 97.4%-79.1% nucleotide homology and 97.2%-83.2% amino acid homology on HN gene. Additional 6 amino acids are encoded by the HN gene ORF of strain YN-PA01 comparing with national reference strains.The studied strain YN-PA01 exhibits highest identities with strain JS/5O1/Go either analyzed on F gene or HN gene.
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关 键 词: | 鹦鹉源I型禽副粘病毒 F-HN基因 序列测定 RT-PCR |
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