| 中国株HIV-1外膜蛋白真核表达载体的构建与表达 |
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| 引用本文： | 王福祥,孙永涛,王临旭,王久平,王平忠,白雪帆,黄长形.中国株HIV-1外膜蛋白真核表达载体的构建与表达[J].Virologica Sinica,2004,19(1):70-72. |
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| 作者姓名： | 王福祥 孙永涛 王临旭 王久平 王平忠 白雪帆 黄长形 |
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| 作者单位： | 第四军医大学唐都医院全军感染病诊疗中心 陕西西安710038
(王福祥，孙永涛，王临旭，王久平，王平忠，白雪帆)，第四军医大学唐都医院全军感染病诊疗中心 陕西西安710038(黄长形) |
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| 摘 要： | To construct the eukaryotic expression vector of HIV-1 gp120 gene and observe its expression in vitro, the recombinant expression vector pVAX1GP120 was constructed by inserting the gp120 gene into the eukaryotic expression vector pVAX1. The pVAX1GP120 was transfected into Vero cells by lipofectamine and the expressed product was detected by indirect immunofluore- scence.Restriction enzymes digestion analysis and sequencing results revealed that the recombinant expression vector pVAX1GP120 has been constructed successfully. The indirect immunofluorescence result showed green fluorescence on the membrane of transfected cells. The constructed eukaryotic expression vector of HIV-1 gp120 can be expressed in vitro, which lay the foundation for the further study of HIV-1 DNA vaccine.
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| 关 键 词： | 外膜蛋白 真核表达载体 HIV-1 获得性免疫缺陷综合征 |
Construction and Expression of HIV-1 Env Eukaryotic Expression Vector |
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| WANG Fu-xiang,SUN Yong-tao,WANG Lin-xu,WANG Jiu-ping,WANG Ping-zhong,BAI Xue-fan,HUANG Chang-xing.Construction and Expression of HIV-1 Env Eukaryotic Expression Vector[J].中国病毒学(英文版),2004,19(1):70-72. |
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| Authors: | WANG Fu-xiang SUN Yong-tao WANG Lin-xu WANG Jiu-ping WANG Ping-zhong BAI Xue-fan HUANG Chang-xing |
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| Institution: | WANG Fu-xiang,SUN Yong-tao**,WANG Lin-xu,WANG Jiu-ping,WANG Ping-zhong,BAI Xue-fan,HUANG Chang-xing |
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| Abstract: | To construct the eukaryotic expression vector of HIV-1 gp120 gene and observe its expression in vitro, the recombinant expression vector pVAX1GP120 was constructed by inserting the gp120 gene into the eukaryotic expression vector pVAX1. The pVAX1GP120 was transfected into Vero cells by lipofectamine and the expressed product was detected by indirect immunofluore- scence. Restriction enzymes digestion analysis and sequencing results revealed that the recombinant expression vector pVAX1GP120 has been constructed successfully. The indirect immunofluorescence result showed green fluorescence on the membrane of transfected cells. The constructed eukaryotic expression vector of HIV-1 gp120 can be expressed in vitro, which lay the foundation for the further study of HIV-1 DNA vaccine. |
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| Keywords: | HIV-1 Envelope protein Eukaryotic expression vector |
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