| 流行性出血热循环免疫复合物组分分析 |
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| 引用本文： | 陈悦,杨东亮.流行性出血热循环免疫复合物组分分析[J].Virologica Sinica,1995,10(2):111-114. |
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| 作者姓名： | 陈悦 杨东亮 |
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| 作者单位： | 同济医科大学附属同济医院临床免疫研究室，湖北省郧阳地区人民医院，安徽省阜阳地区出血热防治研究所 |
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| 摘 要： | 应用ＳＤＳ－聚丙烯酰胺凝胶电泳及免疫转印技术对流行性出血热患才血清中免疫合物组分进行了分析。流行性出血热循环免疫复合物经ＳＤＳ－ＰＡＧＥ分离，考马斯亮兰染色，显色主要有７条带，分子量分别为２３ｋＤ，５０ｋＤ，５２ｋＤ，６５ｋＤ，７２ｋＤ，８０ｋＤ及１００ｋＤ。采用该病毒特异性抗血清、单克隆抗体以及人免疫球蛋白、补体成分抗血清识别，在其特环免疫复合物中可检出特异性病毒抗在及相应的免疫球状蛋白和补体成
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| 关 键 词： | 流行性出血热 循环免疫复合物 补体 |
Ana1ysis of the Components of Circulating Immune Complex in Epidemic Hemorrhagic Fever |
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| Chen Yue,Yang Dongliang,Hao Lianjie,et al..Ana1ysis of the Components of Circulating Immune Complex in Epidemic Hemorrhagic Fever[J].中国病毒学(英文版),1995,10(2):111-114. |
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| Authors: | Chen Yue Yang Dongliang Hao Lianjie |
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| Abstract: | DS-polyacrylamide gel electrophoresis and Western blot analysis were carried out on the components of circulating immune complex(CIC) in sera of patients with epidemic hemorrhagic fever(EHF).After separtion in SDS-PAGE,the cumponents of CIC were stained with coomassie brilliantblue showing mainly 7 bands with appwrant molecular weight about 23kD,50kD,52kD,65kD,72kD,80kD and 100kD,respoctively.EHF virus antigens,human lg and the component of complement couldbe detected in the CIC using the speci fic antibodies against EHF virus,human Ig and C_3,suggesting thatthe EHF virus antigen and human Ig are the main components of the CIC,EHF virus RNA was also detected In CIC from tome patients by RT-PCR,Furthermore,there were at least lgG,IgM,IgA type CICin the sera of EHF potients and most of them were combined with the component of complement. |
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| Keywords: | Epidemic hemorrhagic fever CIC Antigen Antibody Complement |
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