狂犬病毒“北京株”在地鼠肾传代细胞中增殖的电镜观察

用透射电子显微镜观察术，对狂犬病固定毒“北京株”在地鼠肾传代细胞（ＢＨＫ２１）中增殖的形态学变化进行了研究。在受感染的细胞中有大量子弹状和长杆状的病毒颗粒。大多数病毒在扩张的内质网膜上以出芽的方式发生，凸向内质网腔内并逐渐发育成子弹状和杆关的成熟病毒。含病的内质，多周围常伴有颗粒或丝状均质区域，少数病毒在细胞膜上芽生。细胞间隙中亦可见病毒。还见病毒在细胞核膜内、外层上芽生，核周围间隙中许多病毒，有

Morphology study on Fixed Rabies Virus(Beijing Strain)in BHK_(21) Cells by Electron Microscopy

he BHK_21 cells infected with Beijing strain of fixed rabies virus were studied by electron mlcroscopy,The rod-like,bullet,round and oval viruses within the cytoplasm,mostly Iocated in the var-ious cisterna of rough and smooth endoplasmic reticulum and usually associated with homogeneous material masses.The virus may bud from their membranes towards the cisterna,protrude from the cellmembranes or dissociate in extracellular spaces.It was also found that viral formation and growth alsooccured in the nucl eus membranes.Some of the viruses,were located close to inter nucleus membranesand perinuclear chromatin,some damages were also found there.The virus and other features observed above were not seen in uninfected cells,The resuIts show that the rabies virus has a strpng dependence on the membranesystem and a destructive action on BHK_21 cells.