草鱼出血病病毒多肽的免疫原性

采用中和试验比较了草鱼出血病病毒ＧＣＨＶ８７３的１１个多肽的免疫原性，确定了主要中和抗原。纯化的单个病毒多肽免疫家兔获得抗血清，多太ＶＰ１、ＶＰ５、ＶＰ６和ＶＰ９的抗血清具有中和效价，而ＶＰ２、ＶＰ３、ＶＰ４、ＶＰ８、ＶＰ１０和ＶＰ１１则不能诱生中和抗体。其中ＶＰ５的抗血清中和效价最高，因此，ＶＰ６极可能是病毒多肽中的主要中和抗原。

Immunogenicity of Grass Carp Hemorrhage Virus Polypeptides

he immunogenicity of the 11 polypeptides of GCHV873 were commnd by using neutralizationtest for the determination of the major protective antigen.The viral polypeptides labelled with FITCwere analyzed on a 10%polyacrylamide gel.The individual polypeptides were retrieved from the gelby electric elution,and were used for immunizing rabbits to prepare antisera.Each anti-polypeptide antiserum was titrated in a neutralization test.The neutralization titres of the andsera indicated that theneutralizing activity of anti VP_6 antiserum was thehighest among them,while the VP_2,VP_3,VP_4,VP_7,VP_8,VP_(10) and VP_(11) could not induce neutralizing antibodies.Therefore, VP_6 may be a major neutraliring antigen.