| HBV—DNA的生物素寡聚核苷酸探针快速检测 |
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| 引用本文： | 吴云涛,蔡宜权.HBV—DNA的生物素寡聚核苷酸探针快速检测[J].Virologica Sinica,1993,8(4):310-315. |
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| 作者姓名： | 吴云涛 蔡宜权 |
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| 作者单位： | 中国科学院武汉病毒研究所，中国科学院武汉病毒研究所 武汉 430071，武汉 430071 |
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| 摘 要： | 本文建立一种快速ＨＢＶ－ＤＮＡ检测方法，此法是基于生物素标记的寡聚核苷酸与硝酸纤维素滤膜上的靶ＤＮＡ杂交，然后通过Ｓｔｒｅｐｔｏａｖｉｄｉｎ－碱性磷酸酶偶联体及生色底物进行检测。本文研究了此法的实用性，表明对于实验室及临床的ＨＢＶ－ＤＮＡ检测，此法是一种快速（４小时）、灵敏（１－１０ｐｇ）、特异、方便的方法。
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| 关 键 词： | 生物素 探针 乙型肝炎病毒 DNA |
Rapid Detection of HBV-DNA by Using Biotinylated Oligonucleotide Probe |
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| Wu Yuntao Cai Yiquan.Rapid Detection of HBV-DNA by Using Biotinylated Oligonucleotide Probe[J].中国病毒学(英文版),1993,8(4):310-315. |
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| Authors: | Wu Yuntao Cai Yiquan |
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| Abstract: | A rapid method for detection of HBV-DNA was developed. This assay was based on the hybridization of biotinylated oligonucleotide probe to target DNA immobolized on nitrocellulose membrane which was then incubated with avidin-alkaline phosphatase conjugator and measured with chromogenic substrates. The practicality of the method was investigated. It was found that the method was fast (4hr),sensitive(1-10pg)and expedient for laboratory and clinical detection of HBV-DNA. |
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| Keywords: | HBV-DNA DNA Hybridization Biotinylated oligonucleotide |
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