猪瘟病毒E2基因真核表达质粒的构建及基因疫苗的研究

构建了猪瘟（ｃｌａｓｓｉｃａｌ ｓｗｉｎｅ ｆｅｖｅｒ ｖｉｒｕｓ，ＣＳＦＶ）主要保护性抗原Ｅ２基因４种不同的真核表达质粒。小鼠免疫试验表明，Ｅ２基因上不同的功能区对基因疫苗的免疫应答有很大影响，有信号肽序列的Ｅ２基因可诱导产生特异性免疫反应，且无跨膜区序列的Ｅ２基因所诱导的免疫应答反应比有跨膜区序列的强，而无信号肽序列的Ｅ２基因则不能诱导产生ＣＳＦＶ特异性的免疫反应。攻毒保护试验表明，免疫家兔最

Construction of Eukaryotic Expression Plasmids of CSFV E2 Gene and the Study on DNA Vaccine

Four kinds of eukaryotic expressing plasmids of classical swine fever virus (CSFV) E2 gene, either containing or lacking the signal sequence and transmembrane region sequence, were constructed. The antibodies against CSFV were induced after immunization of mice by intramuscular (IM) injection with the plasmids of E2 gene with a signal sequence, pcDST and pcDSW, but not with the plasmids of E2 gene lacking a signal sequence, pcDWT and pcDWW. The level of antibodies elicited by pcDSW lacking transmembrane region sequence was higher than that elicited by pcDST with transmembrane region sequence. The results indicated the plasmid expressing a secreted antigen may be more efficient for induction of immune response when administered by IM injection than that expressing non secreted antigen. Immunization with pcDSW could protect rabbits against challenge with at least 10 MID hog cholera lapinized virus (HCLV) and protect pig from the challenge of lethal dose of CSFV strain, Shimenmen.