| 油桐尺蠖核型多角体病毒DNA聚合酶基因的克隆和序列分析 |
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| 引用本文： | 龚岷,梁布锋.油桐尺蠖核型多角体病毒DNA聚合酶基因的克隆和序列分析[J].Virologica Sinica,2000,15(1):59-65. |
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| 作者姓名： | 龚岷 梁布锋 |
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| 作者单位： | 中国科学院武汉病毒研究所!武汉430071 |
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| 基金项目： | 中国科学院生物区系分类资助的课题 |
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| 摘 要： | 用PCR方法从油桐尺蠖核型多角体病毒 (BusuNPV)中扩增出DNA聚合酶基因片段 ,经pGEM T载体克隆到大肠杆菌DH5α菌株中。经自动序列分析仪测出DNA聚合酶基因 2 379bp长的核苷酸序列 ,推导出 793的氨基酸序列。氨基酸同源性比较显示 ,BusuNPV与HzSNPV的同源性最高 ,达 57% ;与OpMNPV的同源性最低 ,为 39.6 %。
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| 关 键 词： | 油桐尺蠖 核型多角体病毒 DNA聚合酶 |
Cloning and Sequencing of the Buzura suppressaria Nucleopolyhedrovirus DNA Polymerase Gene |
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| GONG Min,LI Wei dong,LIANG Bu feng.Cloning and Sequencing of the Buzura suppressaria Nucleopolyhedrovirus DNA Polymerase Gene[J].中国病毒学(英文版),2000,15(1):59-65. |
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| Authors: | GONG Min LI Wei dong LIANG Bu feng |
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| Abstract: | The DNA polymerase gene of Buzura suppressaria nucleopolyhedrovirus (BusuNPV) was amplified by PCR using virus DNA template. The amplified fragment was cloned into E.coli via plasmid pGEM T. The fragment of insert was analyzed on an automated DNA sequencer. Its sequence showed that the insert has 2 379 nucleotides in length and 793 aa was predicated. Comparing with other six members of Baculoviridae , amaino acid sequence of the DNA polymerase of BusuNPV has 57% similarity with HzSNPV, and 39.6% with OpMNPV. |
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| Keywords: | Buzura suppressaria nucleopolyhedrovirus (BusuNPV) DNA polymerase PCR amplification Sequence analysis |
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