| 水稻瘤矮病毒基因组S9片段的基因结构特征 |
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| 引用本文： | 范国成,吴祖建,黄明华,练君,梁栋,林奇英,谢联辉.水稻瘤矮病毒基因组S9片段的基因结构特征[J].Virologica Sinica,2005,20(5):539-542. |
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| 作者姓名： | 范国成 吴祖建 黄明华 练君 梁栋 林奇英 谢联辉 |
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| 作者单位： | 福建农林大学植物病毒研究所，福建农林大学植物病毒研究所，广东省信宜市农作物病虫测报站，广东省信宜市农作物病虫测报站，广东省信宜市农作物病虫测报站，福建农林大学植物病毒研究所，福建农林大学植物病毒研究所 福建福州350002 福建省农业科学院果树研究所，福建福州350013，福建福州350002，广东信宜525300，广东信宜525300，广东信宜525300，福建福州350002，福建福州350002 |
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| 基金项目： | 国家自然科学基金(39900091) |
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| 摘 要： | 应用RT-PCR技术克隆了水稻瘤矮病毒(RGDV)中国广东信宜分离物(RGDV-C)的基因组S9片段,测定了全序列并进行了生物信息学分析。结果表明,RGDV-C S9片段全长共有1202bp(登录号AY556483),含有一个长的开放阅读框,这一开放阅读框编码一个由323个氨基酸残基组成的多肽,推测分子量约35.6kDa,与泰国分离物(RGDV-T)的全序列相比,它们的核苷酸长度相等,核苷酸同源性为98.1%,氨基酸同源性为98.5%。RGDV S9片段编码的Pns9蛋白在植物呼肠孤病毒属内未发现同源蛋白,其功能尚待确定。利用NCBI的BLAST查找与比较,发现Pns9与伯氏疏螺旋体(Borrelia burgdorferi)ATP依赖的Clp蛋白水解酶组分ATP-dependent Clp prote-ase proteolytic component(clpP-1)]有21.8%的氨基酸序列同源性。
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| 关 键 词： | 水稻瘤矮病毒 基因组S9片段 序列 |
| 收稿时间： | 2005-03-14 |
| 修稿时间： | 2005-05-23 |
Molecular characterization of the genome segment S9 of Rice gall dwarf virus |
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| FAN Guo-cheng, WU Zu-jian , HUANG Ming-hua, LIAN Jun, LIANG Dong, LIN Qi-ying , XIE Lian-hui.Molecular characterization of the genome segment S9 of Rice gall dwarf virus[J].中国病毒学(英文版),2005,20(5):539-542. |
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| Authors: | FAN Guo-cheng WU Zu-jian HUANG Ming-hua LIAN Jun LIANG Dong LIN Qi-ying XIE Lian-hui |
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| Institution: | 1. Institute of Plant Virology, Fujian Agricultural and Forestry University, Fuzhou 350002, China; 2. Pomology Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China ; 3. Station of the Monitoring and Forecasting of Crop Diseases and Pests Xinyi County, Guangdong 525300, China |
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| Abstract: | The full-length cDNA of the genome segment(S9) of Rice gall dwarf virus Xinyi isolate(RGDV-C) was cloned and the complete nucleotide sequence was determined.The results showed that S9 had 1202 nucleotides(AY556483) encoding a polypeptide of 323 amino acids with an Mr of 35.6kDa.The S9 had the same genomic organization as that of RGDV Thailand isolate(RGDV-T) and shared 98.1% nucleotide and 98.5% amino acid sequence identity.No equivalent protein of Pns9 encoded by RGDV S9 ORF was found in other members of the genus Phythoreovirus.Pns9 shared 21.8% amino acid sequence identity with ATP-dependent Clp protease proteolytic component from Borrelia burgdorferi. |
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| Keywords: | Rice gall dwarf virus Genome segment 9 Sequence |
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