| 猪圆环病毒PCR检测方法的优化 |
| |
| 引用本文： | 董志强,张志,李晓成,陈德坤,张燕霞,黄保续.猪圆环病毒PCR检测方法的优化[J].Virologica Sinica,2006,21(4):405-407. |
| |
| 作者姓名： | 董志强 张志 李晓成 陈德坤 张燕霞 黄保续 |
| |
| 作者单位： | 西北农林科技大学，农业部动物检疫所，农业部动物检疫所，西北农林科技大学，农业部动物检疫所，农业部动物检疫所 陕西杨凌712100 农业部动物检疫所，山东青岛266032，山东青岛266032，山东青岛266032，陕西杨凌712100，山东青岛266032，山东青岛266032 |
| |
| 摘 要： | 猪圆环病毒(Porcine circovirus,PCV)为无囊膜的单股负链DNA病毒,是目前发现的最小的动物病毒,大小约为17nm1]。PCV根据抗原性及基因组的不同可以分为PCV1和PCV2两种基因型,PCV1分离自猪肾细胞系PK15,它不致病,能够在PK15中稳定存在而不形成细胞病变;PCV2对猪有致病性,可引起猪断奶后多系统衰竭综合征(PMWS)、猪皮炎与肾病综合征(PDNS)、断奶猪和育肥猪的呼吸道疾病、猪的繁殖障碍等疾病。近年来已成为严重危害我国养猪业的主要疾病之一。但迄今为止市场上还没有商品化疫苗和特效药物防治该病,并且该病有时呈亚临床感染,所以…
|
| 关 键 词： | 圆环病毒 PCR 检测方法 优化 |
| 收稿时间： | 2005-11-19 |
| 修稿时间： | 2006-02-16 |
Optimization of PCR for the Detection of Porcine Circovirus2 |
| |
| DONG Zhi-qiang, ZHANG Zhi, LI Xiao-cheng, CHEN De-kun, ZHANG Yan-xia, HUANG Bao-xu.Optimization of PCR for the Detection of Porcine Circovirus2[J].中国病毒学(英文版),2006,21(4):405-407. |
| |
| Authors: | DONG Zhi-qiang ZHANG Zhi LI Xiao-cheng CHEN De-kun ZHANG Yan-xia HUANG Bao-xu |
| |
| Institution: | 1.North-west Sci-Tech University of Agriculture and Forestry, Yangling 712100, China; 2.National Animal Quarantine Institute of Ministry of Agriculture, Qingdao 266032, China |
| |
| Abstract: | Three pairs of primers P1/P2,P3/P4 and P5/P6 of porcine circovirus 2 were designed and synthesized to compare the sensitivity and specialization to amplify products of 404bp,647bp and 1773 bp.Optimum dilution of PK15 cell genome infected with PCV 2 was achieved with 10-6ng,10-5ng to yield from the three pairs of primers.It was also confirmed that P1/P2 were 68.4% successful in detecting PCV2 in 57 swine samples from the province of Fujian,The other primers,P3/P4,P5/P6,were only 57.9% and 8.7% successful,respectively.We conclude that p1/p2 is the optimum primer pair for surveying incidents of PCV2 in pigs. |
| |
| Keywords: | Porcine circovirus PCR Detection Optimization |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
