| H9N2亚型禽流感病毒非结构蛋白（NS1）基因的克隆与表达 |
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| 引用本文： | 刘金华,吴清民,史为民,郭玉璞.H9N2亚型禽流感病毒非结构蛋白（NS1）基因的克隆与表达[J].Virologica Sinica,2003,18(5):503-505. |
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| 作者姓名： | 刘金华 吴清民 史为民 郭玉璞 |
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| 作者单位： | 中国农业大学农业部预防兽医学重点开放实验室，中国农业大学农业部预防兽医学重点开放实验室，中国农业大学农业部预防兽医学重点开放实验室，中国农业大学农业部预防兽医学重点开放实验室 北京 100094，北京 100094，北京 100094，北京 100094 |
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| 摘 要： | The nostructural protein (NS1) encoded by gene 8 of the Influenza virus is present in cells infected with Influenza virus. In this study, NS1 protein gene of the Chicken influenza virus A/chicken/Beijing/2/97 (H9N2) strain was amplified by PCR. The fragment contains EcoR Ⅰ and Xho Ⅰ restriction enzyme sites at the ends. The amplified product was cloned into the expression vector pET-30(c). Recombinant plasmid pET/NS 1 was transformed into E.coli BL21 (DE3) competent cells and induced with 0.4 mmol/L IPTG the target protein was produced, the molecular weight of the expressed protein was 30 kDa as expected. Western-blot test indicated that the expressed protein can react with the NS 1 monoclonal antibody of the influenza virus. This study laid an important foundation for H9N2 subtype avian influenza surveillance in China.
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| 关 键 词： | 禽流感病毒 非结构蛋白 基因克隆 基因表达 |
Cloning and Expression of the Nonstructural Protein (NS1) of the H9N2 Chicken Influenza Virus |
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| LIU Jin-hua,WU Qing-min,SHI Wei-min,GUO Yu-pu.Cloning and Expression of the Nonstructural Protein (NS1) of the H9N2 Chicken Influenza Virus[J].中国病毒学(英文版),2003,18(5):503-505. |
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| Authors: | LIU Jin-hua WU Qing-min SHI Wei-min GUO Yu-pu |
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| Abstract: | |
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| Keywords: | Chicken influenza virus NS1 Gene expression |
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