| 一种改良的对虾白斑综合征病毒的提纯技术 |
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| 引用本文： | 解云礼,张叔勇,黄茹,石正丽.一种改良的对虾白斑综合征病毒的提纯技术[J].Virologica Sinica,2003,18(4):391-393. |
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| 作者姓名： | 解云礼 张叔勇 黄茹 石正丽 |
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| 作者单位： | 中国科学院武汉病毒研究所分子病毒学重点实验室无脊椎动物病毒学联合开放实验室，中国科学院武汉病毒研究所分子病毒学重点实验室无脊椎动物病毒学联合开放实验室，中国科学院武汉病毒研究所分子病毒学重点实验室无脊椎动物病毒学联合开放实验室，中国科学院武汉病毒研究所分子病毒学重点实验室无脊椎动物病毒学联合开放实验室 湖北武汉 430071，湖北武汉 430071，湖北武汉 430071，湖北武汉 430071 |
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| 基金项目： | 中国科学院生命科学与生物技术领域2001年知识创新工程重要方向项目(KSCX2-SW-302-2)，863海洋生物技术课题(2001AA620201) |
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| 摘 要： | The envelope proteins of White spot syndrome virus (WSSV) are very fragile and easy to be destroyed during purification. It was difficult to obtain a large quantity of intact virions by routine sucrose gradient centrifugation. After modifying the sucrose gradient by adding citrate sodium, we can obtain a large quantity of intact virions and nucleocapsids. This purified virions and nucleocapsids were subsequently used for analyzing viral structural proteins and DNA extraction. The result showed that this modified techniaue is very efficient for virus purification.
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| 关 键 词： | 对虾白斑综合征病毒 提纯 病毒粒子 病毒囊膜 |
A Modified Technique for Purifying White spot syndrome virus |
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| XIE Yun-li,ZHANG Shu-yong,HUANG Ru,SHI Zheng-li.A Modified Technique for Purifying White spot syndrome virus[J].中国病毒学(英文版),2003,18(4):391-393. |
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| Authors: | XIE Yun-li ZHANG Shu-yong HUANG Ru SHI Zheng-li |
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| Institution: | XIE Yun-li,ZHANG Shu-yong,HUANG Ru,SHI Zheng-li~ |
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| Abstract: | The envelope proteins of White spot syndrome virus (WSSV) are very fragile and easy to be destroyed during purification. It was difficult to obtain a large quantity of intact virions by routine sucrose gradient centrifugation. After modifying the sucrose gradient by adding citrate sodium, we can obtain a large quantity of intact virions and nucleocapsids. This purified virions and nucleocapsids were subsequently used for analyzing viral structural proteins and DNA extraction. The result showed that this modified technique is very efficient for virus purification. |
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| Keywords: | White spot syndrome virus (WSSV) Sucrose gradient centrifugation Purification technique |
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