猪细小病毒SD-68株NS1基因的克隆与序列分析

对猪细小病毒(PPV)SD-68株NS1基因进行了克隆和序列测定，结果表明SD-68株NS1基因全长1989bp，编码662个氨基酸组成的多肽。该序列与PPV SY-99株、Kresse株、NADL-2(5075)和NADL-2(4973)株的NS1基因比较，核苷酸的同源性分别为99．9％、99．9％、99．7％、98．1％，氨基酸的同源性分别为99．7％、99．5％、99．5％、96．7％。PPV SD-68株与MVM(i)、MEV(Abashiri)、CPV、FPV、BPV3、GPVNSl的进化树分析表明：PPVSD-68株NS1与MVM(i)NS1亲缘关系最近，与BPV3 NS1的亲缘关系最远；在Thr435和Ser473位点PPV SD-68株与MVM(i)完全一致，表明Thr435和Ser473是PPV SD-68株NS1潜在的磷酸化位点。

Cloning and Sequence Analysis of NS1 Gene of Porcine Parvovirus SD-68 Strain

NS1 gene of Porcine parvovirus SD-68 strain was cloned and sequenced. Based on the sequence we can conclude that NS1 gene of SD-68 strain is composed of 1989 nucleotides, encodes a polypiptide of 662 amino acids and shares 99.9%, 99.9%, 99.7%, 98.1% identity of nucleotides and 99.7%, 99.5%, 99.5%, 96.7% identity of amino acids with that of SY-99 strain, Kresse strain, NADL-2(5075) strain and NADL-2(4973) strain, respectively. The distances of NS1 gene of all strains analyzed by phylogenetic tree, we can conclude that SD-68 strain is near to MVM(i) strain and far to BPV3 strains. The residues of Thr and Ser at the position 435 and 473 in the NS1 gene of SD-68 strain are phosphorylated sites because they are also found at the same position in the NS1 gene of MVM(i) strain.