| 油桐尺蠖卵巢细胞系BS-484克隆株特性的研究 |
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| 引用本文： | 梁布锋,梁莉,邹帅洲.油桐尺蠖卵巢细胞系BS-484克隆株特性的研究[J].Virologica Sinica,1989(4). |
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| 作者姓名： | 梁布锋 梁莉 邹帅洲 |
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| 作者单位： | 中国科学院武汉病毒研究所 武汉 |
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| 基金项目： | 中国科学院青年科学基金资助的课题 |
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| 摘 要： | 通过有限稀释法由Bs—484细胞系中成功地分离出四个克隆株(Bs—484B,E,F,G)。克隆细胞侏的生长特性不同于原细胞株Bs—484,各克隆株之间的形态特征、细胞倍增时间、以及在维持油桐尺蠖核型多角体病毒的复制能力等方面均有差异。用三种同工酶(乳酸脱氢酶、苹果酸脱氢酶和酯酶)比较了各克隆株与原细胞株之间的异同。
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| 关 键 词： | 昆虫细胞系 同工酶 克隆 油桐尺蠖核型多角体病毒 |
Characterization of Clonal Strains from the Buzura suppressaria Ovary Cell Line Bs-484 |
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| Liang Bu-fpng Liang Li Zou Shuai-zhou.Characterization of Clonal Strains from the Buzura suppressaria Ovary Cell Line Bs-484[J].中国病毒学(英文版),1989(4). |
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| Authors: | Liang Bu-fpng Liang Li Zou Shuai-zhou |
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| Abstract: | Four clones (Bs-484 B,E, F, G) were successfully isolated from the established cell line of Bs-484 by the method of finite dilution. The growth characteristics of the clonal strains were different from the parent line. The cloned cell strains were different in their predominant morphology, cell doubling times and the relative ability to support replication of the nuclear polyhedrosis virus of Buzura suppressaria (BsNPV). The origin of the cloned cell strains was confirmed by comparing their isozyme profiles with the parental Bs-484 cell line using lactate dehydrogenase (LDH), malic dehydrogenase (MDH) and esterase (EST). |
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| Keywords: | Insect cell line Isozyme Clones BsNPV |
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