首页 | 本学科首页   官方微博 | 高级检索  
   检索      

IL—18DNA免疫对HIV—1核酸疫苗诱导的免疫应答的影响
引用本文:王福祥,孙永涛,孙永年,徐哲,王临旭,刘娟,康文臻.IL—18DNA免疫对HIV—1核酸疫苗诱导的免疫应答的影响[J].Virologica Sinica,2004,19(2):97-100.
作者姓名:王福祥  孙永涛  孙永年  徐哲  王临旭  刘娟  康文臻
作者单位:第四军医大学唐都医院全军感染病诊疗中心 陕西西安710038 (王福祥,孙永涛,孙永年,徐哲,王临旭,刘娟),第四军医大学唐都医院全军感染病诊疗中心 陕西西安710038(康文臻)
摘    要:为了研究白细胞介素-18(IL-18)基因对人免疫缺陷病毒(HIV-1)核酸疫苗诱导免疫应答的影响,将人IL-18基因插入到真核表达载体pVAX1中,构建了真核表达载体pVAX1-IL-18;将pCI-neoGAG联合pVAX1-IL-18或者pCI-neoGAG单独免疫Balb/c小鼠,检测免疫小鼠的特异性抗体和IFN-γ,同时观察免疫小鼠脾淋巴细胞增殖和小鼠特异性细胞毒性T淋巴细胞(CTL)反应。酶切及测序结果表明成功地构建了人IL-18基因真核表达载体;与pCI-neoGAG免疫组比较,pCI-neoGAG联合pVAX1-IL-18免疫组小鼠血清的抗HIV-1p24抗体滴度降低(P<0.01);而与pCI-neoGAG免疫组比较,pCI-neoGAG联合pVAX1-IL-18免疫组小鼠血清的IFN-γ升高(P<0.01);pCI-neoGAG联合pVAX1-IL-18免疫组小鼠的脾淋巴细胞增殖实验刺激指数(SI)以及特异性CTL活性均高于pCI-neoGAG免疫组(P<0.01)。IL-18基因联合HIV-1核酸疫苗免疫小鼠,可能增强特异性Th1细胞和CTL反应,白细胞介素-18基因对体液免疫有抑制作用。

关 键 词:白细胞介素-18  IL-18  基因  人免疫缺陷病毒  HIV-1  核酸疫苗  免疫

Modulation of Immune Responses to a HIV-1 Nucleic Acid Vaccine by Interleukin-18 DNA Immunization
WANG Fu-xiang,SUN Yong-tao,SUN Yong-nian,XU Zhe,WANG Lin-xu,LIU Juan,KANG Wen-zhen.Modulation of Immune Responses to a HIV-1 Nucleic Acid Vaccine by Interleukin-18 DNA Immunization[J].中国病毒学(英文版),2004,19(2):97-100.
Authors:WANG Fu-xiang  SUN Yong-tao  SUN Yong-nian  XU Zhe  WANG Lin-xu  LIU Juan  KANG Wen-zhen
Institution:WANG Fu-xiang,SUN Yong-tao**,SUN Yong-nian,XU Zhe,WANG Lin-xu,LIU Juan,KANG Wen-zhen
Abstract:To investigate the effect of interleukin-18 (IL-18) DNA immunization on immune response induced by Human immunodeficiency virus 1(HIV-1) nucleic acid vaccine (DNA vaccine), the recombinant expression vector pVAX1-IL-18 was constructed by inserting the IL-18 gene into the eukaryotic expression vector pVAX1. Balb/c mice were immunized with pCI-neoGAG alone or co-administered with the DNA encoding for IL-18. Their sera were collected for analyzing anti-HIV antibody and IFN-?by ELISA, and spleen cells were isolated for detecting antigen-specific lymphoproliferative responses and specific CTL response by MTT assay and LDH assay, respectively. Restriction enzymes digestion analysis and DNA sequencing results revealed that the recombinant expression vector pVAX1-IL-18 has been constructed successfully. The anti-HIV antibody titers of mice co-immunized with pCI-neoGAG and the DNA encoding for IL-18 were lower than that of mice immunized with pCI-neoGAG alone (P<0.01). In contrast, the IFN- level of mice co-immunized with pCI-neoGAG and the DNA encoding for IL-18 was higher than that of mice immunized with pCI-neoGAG alone (P<0.01). Furthermore, compared with mice injected with pCI- neoGAG alone, the specific CTL cytotoxity and antigen-specific lymphoproliferative responses of mice immunized with pCI-neoGAG and the DNA encoding for IL-18 were significantly enhanced (P<0.01). The DNA encoding for IL-18 together with HIV DNA vaccine may enhance specific Th-1 responses and cellular immune responses elicited in mice. However, the DNA encoding for IL-18 may down-regulate the humoral responses.
Keywords:Human immunodeficiency virus 1(HIV-1)  DNA vaccination  Interleukin-18(IL-18)  Immunization  
本文献已被 CNKI 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号