用DNA银染法研究传染性软疣病毒的形态发生发育过程

用ＤＮＡ银染技术显示了传染性软疣病毒（ＭＣＶ）形态发生发育及其过程中ＤＮＡ的变化。结果显示：在被感染的皮肤表皮细胞内都有一个大小及构型不同的银染区（病毒工厂）。ＭＣＶ的发生发育均在银染区内而不在胞质区内。其发生过程是先在细胞一侧的胞质内复制合成病毒ＤＮＡ等物质，形成中等密度的银粒大小不等的银染区（病毒前包涵体区），然后在其中形成致密的细粒状银染区（病毒前基质区），接着在后者的周围出现弧形的粗粒银沉淀（初期ＭＣＶ的外膜），逐渐分割包围病毒前基质而形成初期ＭＣＶ。在发育过程中，初期ＭＣＶ的外膜、基质，核心外膜及核心均经过一系列的形态变化。侧体是中期ＭＣＶ向成熟期发育中出现的暂时性结构，其本质为含ＤＮＡ成分的病毒基质。本研究提示：ＭＣＶ的ＤＮＡ物质进入皮肤表皮细胞后能大量复制，合成大量的病毒蛋白质，自主地装配成完整的初期ＭＣＶ，后者有独特的形态发育过程。

Study on Morphogenesis and Development Process of MCV by Silver-DNA Staining and Electron Microscopy

fter using the methenamine silver-DNA staining,15 cases of eariy molluscum contagiosumtissues were examined by electron microscopy.The results showed that there was a silver-stainedDNA area(inclusion body,IB)in every cell infected with MCV. The morphogenesis and develop-ment of MCV tcok place in the IB,not in the cytoplasm area. The process of MCV morphogenesiswas firstly to synthesize virus materials(DNA) which secondly accumulated into a filament-granu-lar-like area(previrus inclusion body,PIB),and next to form some dense thin-granular-chain-likesturctures(previrus matrix,PM)in PIB,and then the bilayer membranes which cut and surroundPM to form primary MCV. The development process was from primary to intermediate,then tomatural and finally to degenerate MCV. And in the process the outer membrane,matrix,nucleusand the nuclear outermembrane of MCV had many changes.The lateral bodies were the temporalstructures and the viroplasm containing DNA in the intermediate MCV.The results suggested thatthe MCV gene may spontaneously synthesize virus DNA,form previrus matrix and virus outer-membranes and assemble primary MCV in the IB.