| 鸡传染性法氏囊病上海超强毒株NH99的分离与鉴定 |
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| 引用本文： | 孙建和,陆苹,等.鸡传染性法氏囊病上海超强毒株NH99的分离与鉴定[J].Virologica Sinica,2002,17(3):252-256. |
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| 作者姓名： | 孙建和 陆苹 |
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| 作者单位： | 上海交通大学农业与生物学院生物技术研究所 上海201101
(孙建和，陆苹，李晖)，上海交通大学农业与生物学院生物技术研究所 上海201101(赵渝) |
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| 基金项目： | 上海市科学技术发展基金资助项目 (0 1JC14 0 3 4) |
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| 摘 要： | 通过对上海近郊某鸡场数群 10日龄左右的病鸡的临床症状、病理变化、病毒分离、纯化、动物回归、血清学检测及病毒核酸纯化、VP2基因序列的测定与分析 ,确认上海地区以发病日龄早 ,发病率、死亡率高为特征的鸡传染病为超强毒型鸡传染性法氏囊病 ,病原具有鸡传染性法氏囊病病毒超强毒 (vvIBDV)的分子特征 ,为vvIBDV。
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| 关 键 词： | 鸡传染性法氏囊病病毒超强毒(vvIBDV) 病毒提纯 动物回归 序列分析 |
Isolation and Identification of Very Virulent Infectious Bursal Disease Virus(vvIBDV) Strain NH99 |
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| Abstract: | An infectious bursal disease virus(IBDV) field strain, named NH99, was isolated and identified from some flocks with immune failure in Shanghai according to the clinical symptom, pathology, serological test, animal challenge and virus morphology. The virus was characterized as a very virulent infectious bursal disease virus(vvIBDV) through nucleic acid isolation and related DNA sequence analysis which have the same molecular characteristics as vvIBDV. This is the first report of outbreak of acute infectious bursal disease caused by vvIBDV in Shanghai. |
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| Keywords: | Very virulent infectious bursal disease virus(vvIBDV) Animal challenge Virus purification DNA sequence analysis |
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