中国不同地区和人群HDV cDNA的筛选、克隆及抗原编码区基因异质性的分析

为研究我国不同地区不同人群中ＨＤＶ毒株的感染分子特征，从我国河南、内蒙、北京、四川、广西、西藏、新疆、辽宁、上海等地的ＨＤＶ健康携带者、慢性丁肝病人与重症肝炎病人中筛选获得１０余份ＨＤＶ－ＲＮＡ阳性血清。经逆转录一多聚酶链反应（ＲＴ－ＰＣＲ）交叉扩增获得ＨＤＶ抗原编码区的ｃＤＮＡ片段并克隆到ＰＧＥＭ－３Ｚｆ（－）或ＰＧＥＭ－Ｔ载体上，经序列分析研究其基因结构特点，结果表明：中国的ＨＤＶ毒株基因型均为Ⅰ型，但至少存在ⅠＡ、ⅠＢ两个亚型，ＨＤＶ毒株在不同地区间存在异质性，其中河南－１、－２、－３株及新疆株与台湾株同源性较高（核苷酸与氨基酸同源性分别大于９２．１％与８６．９％）．当为ⅠＡ型；内蒙－１、四川、广西、西藏－１、辽宁、北京株与美国－１株同源性较高（核苷酸与氨酸同源性分别大于９４．３％与８８．８％），当为ⅠＢ亚型；上海株与意大利株的核昔酸同源性最高，为９８．１％。研究证明我国新疆、内蒙、西藏等地区抗ＨＤ阳性率比其他省市高并不是由于存在其他基因型所致。

Screening Cloning and Genetic Heterogeneity of the Hepatitis Delta Virus Antigen-Coding Regions Isolated from Different Areas and Population in China

ore than ten Chinese hepatitis,delta virus isolates were coml,ared by sequencing the antigen-ceding regions after cloned to PGEM-3Zf(-)or PGEM-Tvector.The viruses compared in-cluded the Henan-1,Henan-2,Henan-3,Xinjiang ,Neimeng,Sichuan,Xizang,Liaoning,Beijing,Guangxi and Shanghai strains derived from asymptomatic carriers and chronic or fulminant hepatitis patients.It was found that all HDV isolates prevalente in China were genotype I but there wereat least two subgroups. The genet ic heterogeneity of HDAg-coding regions existed among theHDV isolates from different areas or population.These strains from Henan and Xinjiang areasshared over 92.1%nucleotide and 86.9%amino acid homology with Taiwan strain that repre-sents genotype IA.But the strains from Sichuan,Xizang-1,Neimeng-1,Liaoning,Beijing andGuangxi are closely related to US-1strain representing genotype IB(over 94.3%and 88.8%i-dentity in the nucleotide and amino acid sequences respectively).The Shanghai strain is closely re-lated to Italy strain and over 98.1%identity in nucleotide sequences.The results indicated thatthe higher anti-HDV rates in Xinjiang,Neimeng and Xizang areas than in other provinces are notcaused by another special HDV genotype.