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流式细胞术检测汉坦病毒抗原血症的临床应用
引用本文:王卫华,王峰,陈洁,汪丽,邹循亮,顾欣,周彬雷,于宏升.流式细胞术检测汉坦病毒抗原血症的临床应用[J].Virologica Sinica,2006,21(3):298-300.
作者姓名:王卫华  王峰  陈洁  汪丽  邹循亮  顾欣  周彬雷  于宏升
作者单位:宁波大学医学院附属医院检验科,宁波市医疗中心李惠利医院检验科,宁波大学医学院附属医院检验科,宁波大学医学院附属医院检验科,宁波大学医学院附属医院检验科,宁波市中心血站,宁波大学医学院附属医院检验科,Dart Mouth Medical College USA,浙江宁波315020,浙江宁波315010,浙江宁波315020,浙江宁波315020,浙江宁波315020,浙江宁波315010,浙江宁波315020
基金项目:宁波市医学科技项目(2003034).
摘    要:在我国,肾综合征出血热(HFRS)是严重威胁人民身体健康乃至生命安全的急性传染病。及时有效的实验室检查对于指导临床尽早实施抗病毒治疗、进行预后评估,从而减轻患者临床症状、改变病程甚至降低死亡率都有极其重要的意义。本研究旨在建立流式细胞术定量检测外周血单个核细胞(PB

关 键 词:流式细胞术  肾综合征出血热  抗原  汉坦病毒
收稿时间:2005-11-14
修稿时间:2006-03-06

Clinical Application of Flow Cytometry for Quantification of Hanta Virus Antigenemia in Patients with Hemorrhagic Fever with Renal Syndrome
WANG Wei-hua, WANG Feng, CHEN Jie, WANG Li, ZHOU Xun-liang, GU Xin, ZHOU Bin-Lei, YU Hong-shen.Clinical Application of Flow Cytometry for Quantification of Hanta Virus Antigenemia in Patients with Hemorrhagic Fever with Renal Syndrome[J].中国病毒学(英文版),2006,21(3):298-300.
Authors:WANG Wei-hua  WANG Feng  CHEN Jie  WANG Li  ZHOU Xun-liang  GU Xin  ZHOU Bin-Lei  YU Hong-shen
Institution:1.Clinical Laboratory Center, Affiliated Hospital of Medical College, Ningbo University, Ningbo 315020, China; 2.Medical Center of Ningbo City, Lihuili Hospital, Ningbo 315010, China; 3.Blood Center of Ningbo, Ningbo 315010, China; 4.Dart Mouth Medical College, USA
Abstract:The clinical value of the detection Hanta virus antigenemia in Peripheral blood mononuclear cells (PBMC) in patients with Hemorrhagic fever with renal syndrome was evaluated. Samples from 32 cases with varying degrees of HFRS were subjected to quantitative detection for HV membrane antigen in PBMC by flow cytometry. The correlation between clinical severity and positive rates was analyzed. On the first day of onset of illness, positive PBMC rates were highly different from group to group (P<0.01), which suggested a close relationship between clinical severity and the positive rate. Dynamic observations showed that in the light and moderate groups, positive rates differed significantly from day to day. This was not the case in the severe group with no statistical significance between the 7th and 10th day(P>0.05), which implied a relatively slow rate of virus elimination. Quantitative analysis of HV antigen in PBMC by FCM is a promising method in early diagnosis, individualized therapy and in evaluating the prognosis of HFRS.
Keywords:Flow cytometry  Hemorrhagic fever with renal syndrome  Antigen  Hantavirus  
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