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云南省4种伊蚊的乙型脑炎病毒分离物的研究
引用本文:张海林,施华芳.云南省4种伊蚊的乙型脑炎病毒分离物的研究[J].Virologica Sinica,1999,14(1):32-34.
作者姓名:张海林  施华芳
作者单位:云南省流行病防治研究所
摘    要:在云南省西南边境9县市捕获伊蚊属雌性成蚊16种19367只,用细胞法和乳鼠法分离病毒。从185批6491只白纹伊蚊中分离到病毒2株,从50批1605只剌扰伊蚊中分离到病毒2株,从23批772只窄翅伊蚊中分离到病毒2株,从4批103只阿萨姆伊蚊中分离到病毒1株。其它12种共10396只伊蚊的病毒分离物为阴性。分离到的7株病毒经免疫荧光、酶免疫、血凝抑制和中和试验鉴定,均为乙型脑炎病毒(JEvirus)。白纹伊蚊是野外竹林的优势蚊种。分析认为白纹伊蚊在当地乙型脑炎病毒保存和传播中起重要作用,刺扰伊蚊、窄翅伊蚊和阿萨姆伊蚊亦可参与该病毒的传播。

关 键 词:蚊类,伊蚊属,流行性乙型脑炎病毒,媒介,云南省

Isolation of Japanese Encephalitis Virus from Four Species of Aedes Mosquito in Yunnan Province
Zhang Hailin,Shi Huafang,Mi Zhuqing,Zi Dengyun,Gong Zhengda.Isolation of Japanese Encephalitis Virus from Four Species of Aedes Mosquito in Yunnan Province[J].中国病毒学(英文版),1999,14(1):32-34.
Authors:Zhang Hailin  Shi Huafang  Mi Zhuqing  Zi Dengyun  Gong Zhengda
Abstract:A total of 19 367 adult female mosquitoes belonging to 16 species of Aedes genus were captured from nine countries, south western part of Yunnan Province, China, and were examined by C6/36 cell method and suckling mouse method. Two strains of Japanese encephalitis (JE) virus were isolated from 6491 (185 pools) Aedes albopictus , two strains of JE virus were isolated from 1605 (50 pools) Ae. vexans, two strains of JE virus were isolated from 772 (23 pools) Ae. lineatopennis, and a strain of JE virus was isolated from 103 (4 pools) Ae. assamensis. Isolation of JE virus was negative to other 12 species of Aedes mosquitoes. Ae. albopictus was the superior species of mosquitoes in bamboo forest. These results indicated that Ae. albopictus from Yunnan has a potential role in the maintenance and transmission of JE virus in nature. Ae. vexans, Ae. lineatopennis and Ae. assamensis are also considered to be transmitting vector of JE virus in Yunnan.
Keywords:Mosquito    Aedes    Japanese encephalitis virus  Vector  Yunnan province
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