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非洲猪瘟病毒j5R膜蛋白的电脑预测和实验证实
引用本文:孙怀昌.非洲猪瘟病毒j5R膜蛋白的电脑预测和实验证实[J].Virologica Sinica,1999,14(3):236-243.
作者姓名:孙怀昌
作者单位:扬州大学畜牧兽医学院动医系!扬州225009,InstituteforAnimalHealth!PirbrightLaboratory,AshRoad,Pirbright,SurreyGU24ONF,UK,InstituteforAnimalHealth!PirbrightLaboratory,AshRoad,Pirbright,SurreyGU24ONF,UK
基金项目:英国生物技术及生物科学研究院和农业部资助
摘    要:蛋白多肽二级结构的电脑预测表明,非洲猪瘟病毒( African swine fever virus , A S F V)j5 R阅读框编码12 .9 k Da 膜蛋白。该蛋白的 C 末端含有一个潜在抗原决定簇,针对其合成肽的抗体能在 A S F V 感染细胞和病毒颗粒中检测到23 或25 k Da( 取决于不同毒株) 特异蛋白。免疫荧光试验显示,j5 R 蛋白主要位于感染细胞的病毒复制部位。油水两相分离和细胞分级分离试验结果证明j5 R 蛋白是膜相关蛋白

关 键 词:非洲猪瘟病毒  j5R阅读框  膜蛋白

Computer Based Prediction and Experimental Confirmation of the j5R Membrane Protein of African Swine Fever Virus
Sun Huaichang.Computer Based Prediction and Experimental Confirmation of the j5R Membrane Protein of African Swine Fever Virus[J].中国病毒学(英文版),1999,14(3):236-243.
Authors:Sun Huaichang
Abstract:The j5R open reading frame (ORF) of African swine fever virus was predicted by computing to encode a 12.9 kDa protein with two successive N terminal transmembrane domains and one C terminal antigenic epitope. Antibodies raised against a synthetic peptide derived from the C terminal epitope detected a specific protein of 23 or 25 kDa (depending on different isolates) in ASFV infected cells or purified extracellular ASFV virions. Immunofluorescence showed that the j5R protein was mainly located in the virus assembly sites within ASFV infected cells. Phase separation of purified extracellular ASFV virions and fractionation of ASFV infected cells demonstrated that the j5R protein was in the detergent phase and membrane fraction, confirming that the j5R protein was membrane associated.
Keywords:African swine fever virus(ASFV)  j5R ORF  Membrane protein
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