| 汉滩病毒S片段基因编码区在Vero—E6细胞中的表达 |
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| 引用本文： | 张梦华,王航雁.汉滩病毒S片段基因编码区在Vero—E6细胞中的表达[J].Virologica Sinica,1999,14(2):181-184. |
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| 作者姓名： | 张梦华 王航雁 |
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| 作者单位： | 第四军医大学唐都医院传染科 |
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| 摘 要： | 汉滩病毒属于布尼亚病毒科汉坦病毒属，主要引起人类肾综合征出血热［１］。我国是肾综合征出血热发生和流行的主要疫区，该病起病急、病死率高，流行范围广，因此寻找安全有效、低廉的疫苗是控制本病流行和发生的关键。基因免疫是近年来微生物学和免疫学研究的新热点［２...
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| 关 键 词： | 汉滩病毒，基因表达，S片段，编码区 |
Transient Expression of Hantaan Virus S segment Gene Coding region in vitro of Vero E 6 cells |
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| Zhang Menghua,Wang Hangyan,Yang Weisong,Huang Changxing,Li Guangyu,Wang Yi,Bai Xuefan.Transient Expression of Hantaan Virus S segment Gene Coding region in vitro of Vero E 6 cells[J].中国病毒学(英文版),1999,14(2):181-184. |
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| Authors: | Zhang Menghua Wang Hangyan Yang Weisong Huang Changxing Li Guangyu Wang Yi Bai Xuefan |
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| Abstract: | The coding region of S genome segment of Hantaan virus (76/118 strain) was inserted into the eukarytic expression plasmidpVR1012. The recombinant expression plasmid pVRS 22 was constructed. Vero E 6 cells were transiently transfected in vitro with pVRS 22 plasmid. The transient expression of Hantaan virus nucleocapsid proteins in Vero E 6 cells was detected by indirect immunofluorescence assay (IFA) with monoclonal antibody 5H5 against Hantaan virus. |
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| Keywords: | Hantaan virus Gene expression S segment Coding region |
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