| 检测猪繁殖与呼吸综合征抗体的重组N蛋白-乳胶凝集试验的建立 |
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| 引用本文： | 许立华,苏鑫铭,刘华雷,任雪枫,王志亮,吴延功,陈溥言.检测猪繁殖与呼吸综合征抗体的重组N蛋白-乳胶凝集试验的建立[J].Virologica Sinica,2004,19(4):401-403. |
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| 作者姓名： | 许立华 苏鑫铭 刘华雷 任雪枫 王志亮 吴延功 陈溥言 |
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| 作者单位： | [1]南京农业大学农业部畜禽疫病诊断与免疫重点开放实验室，江苏南京210095 [2]农业部动物检疫所，山东青岛266032 |
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| 基金项目： | 国家“863”高技术发展计划资助项目(2001AA249012) |
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| 摘 要： | The purified recombinant nuclocapsid protein of PRRSV was used to conjoined with latex,and the conjoined concentration, temperature and time were optimized, a detection method for serum antibodies against Porcine reproductive and respiratory syndrome virus was established. Some tests for the method's sensitivity, specificity and stability were confirmed. About 200 serum samples were detected by using the method, and these samples were also detected by recombinant N protein based ELISA and IDEXX ELISA kit. The result showed that the agreement ratio of the recombinant N protein based LAT with other two methods arrived at 93%. 78%. respectively.
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| 关 键 词： | 重组N蛋白 猪繁殖与呼吸综合征 抗体 乳胶凝集试验 PRRS 猪繁殖与呼吸综合征病毒 |
Establishment of Recombinant N Protein Based Latex Agglutination Test for Detection of Antibody Against Porcine Reproductive and Respiratory Syndrome Virus |
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| XU Li-hua,SU Xin-ming,LIU Hua-lei,REN Xue-feng,WANG Zhi-liang, WU Yan-gong,CHEN Pu-yan.Establishment of Recombinant N Protein Based Latex Agglutination Test for Detection of Antibody Against Porcine Reproductive and Respiratory Syndrome Virus[J].中国病毒学(英文版),2004,19(4):401-403. |
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| Authors: | XU Li-hua SU Xin-ming LIU Hua-lei REN Xue-feng WANG Zhi-liang WU Yan-gong CHEN Pu-yan |
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| Institution: | XU Li-hua1,2,SU Xin-ming1,LIU Hua-lei1,REN Xue-feng1,WANG Zhi-liang,3 WU Yan-gong,CHEN Pu-yan1** 3 |
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| Abstract: | |
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| Keywords: | Recombinant N protein Antibodies of porcine reproductive and respiratory syndrome Serum antibody Latex agglutination test |
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