| IBDVvp2基因高变区序列测定与进化分析 |
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| 引用本文： | 闫笑,李天宪,范兆军,寇铮,陈绳亮.IBDVvp2基因高变区序列测定与进化分析[J].Virologica Sinica,2004,19(6):620-623. |
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| 作者姓名： | 闫笑 李天宪 范兆军 寇铮 陈绳亮 |
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| 作者单位： | 中国科学院武汉病毒研究所 湖北武汉430071
(闫笑，李天宪，范兆军，寇铮)，中国科学院武汉病毒研究所 湖北武汉430071(陈绳亮) |
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| 基金项目： | 国家科技攻关计划项目(2004BA519A11) |
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| 摘 要： | 本实验采用来源于江苏地区的六株不同鸡传染性法氏囊病病毒(Infectious bursal disease virus，IBDV)毒株，应用RT-PCR法对vp2基因高变区进行了扩增，构建重组质粒pMD18T-vp2，测序。与有代表性的IBDV毒株VP2基因高变区序列进行比较分析，以ClustalX软件进行序列比对，得到基因序列及氨基酸序列同源性，IBDV Y3、P2G、P8G、SZ、Y5和W04(6株)IBDV与D6984(荷兰)的同源性达到99．0％以上，与其它一些超强毒株(very virulent IBDV，vvIBDV)的同源性也达到了97．8％以上，并在关键氨基酸位点符合vvIBDV特征，采用Phylip3．5软件分析作出进化树，其结果从分子水平说明六个毒株均为vvIBDV，与欧洲和日本的超强毒株有较近的亲缘关系，而与美洲株的较远，从而为IBDV分子流行病学研究和疫苗的研制提供了科学依据。
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| 关 键 词： | 鸡传染性法氏囊病病毒（IBDV） vp2基因 高变区 超强毒株 |
Sequencing and Phelogenetic Analysis of Hypervariable Region of Infectious Bursal Disease Virus vp2 Gene |
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| YAN Xiao,LI Tian-xian,FAN Zhao-jun,KOU Zheng,CHEN Sheng-liang.Sequencing and Phelogenetic Analysis of Hypervariable Region of Infectious Bursal Disease Virus vp2 Gene[J].中国病毒学(英文版),2004,19(6):620-623. |
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| Authors: | YAN Xiao LI Tian-xian FAN Zhao-jun KOU Zheng CHEN Sheng-liang |
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| Institution: | YAN Xiao,LI Tian-xian**,FAN Zhao-jun,KOU Zheng,CHEN Sheng-liang |
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| Abstract: | Hypervariable region of vp2 gene of six different IBDV (Infectious bursal disease virus) isolates, which were isolated from Jiangsu province, was amplified by RT-PCR and sequenced. Furthermore, sequences of six IBDV isolates were analyzed by ClustalX and Phylip3.5. It was suggested that three isolates (Y3, P2G, P8G) were closely related to vvIBDV (very virulent IBDV) strains isolated from China and Europe. Other isolates (SZ, Y5, W04) were very close with Japanese vvIBDV strains. This study laid on foundation for the research of molecular epidemiology and the development of IBDV vaccines. |
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| Keywords: | Infectious Bursal Disease Virus(IBDV) vp2 gene Hypervariable region Very virulent IBDV |
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