| 猪瘟病毒结构蛋白E~(rns)在E.coli中的高效表达及纯化 |
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| 引用本文： | 尹双辉,刘湘涛,韩雪清,尚佑军,孙士琪.猪瘟病毒结构蛋白E~(rns)在E.coli中的高效表达及纯化[J].Virologica Sinica,2006(1). |
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| 作者姓名： | 尹双辉 刘湘涛 韩雪清 尚佑军 孙士琪 |
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| 作者单位： | 中国农业科学院兰州兽医研究所农业部畜禽病毒学重点实验室 甘肃兰州730046 |
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| 基金项目： | 国家“863”高新技术研究发展计划资助项目(2003AA241110) |
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| 摘 要： | 猪瘟(Classical swine fever,CSF)是由猪瘟病毒(Classical swine fever virus,CSFV)引起的猪的高度接触性传染病,是严重危害养猪业的传染病之一。CSFV基因组为单股正链RNA分子,长约12.3kb,仅编码一个开放性阅读框。位于5′端的囊膜糖蛋白Erns、E1和E2构成了CSFV的外壳,其中Erns
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| 关 键 词： | 猪瘟病毒 Ernsgene 蛋白表达 |
Expression and Purification of E~(rns) Gene of Classical Swine Fever Virus in E.coli |
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| YIN Shuang-hui,LIU Xiang-tao,HAN Xue-qing,SHANG You-jun,SUN Shi-qi.Expression and Purification of E~(rns) Gene of Classical Swine Fever Virus in E.coli[J].中国病毒学(英文版),2006(1). |
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| Authors: | YIN Shuang-hui LIU Xiang-tao HAN Xue-qing SHANG You-jun SUN Shi-qi |
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| Abstract: | The Erns gene was amplified by RT-nPCR from C-strain of Classical swine fever virus(CSFV).Then the Erns gene was cloned into pGEX6P-1 vector.The expression products were analysed by SDS-PAGE and Western blotting methods.The inclusion bodies were recovered from bacterial lysate by centrifugation and washed with buffer,and then dissolved in denaturing agents.The protein was refolded by dilution dialysis.Refold protein reacted strongly with the positive serum of CSFV,which indicated that the protein has immunogenic. |
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| Keywords: | CSFV Erns gene Protein expression |
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