| 人工合成多肽检测抗HIV－1和HIV－2抗体的研究 |
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| 引用本文： | 洪世雯,何红霞,朱风兰,林涛.人工合成多肽检测抗HIV－1和HIV－2抗体的研究[J].Virologica Sinica,1995(3). |
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| 作者姓名： | 洪世雯 何红霞 朱风兰 林涛 |
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| 作者单位： | 中国人民解放军三０二医院 |
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| 摘 要： | 采用固相法合成ＨＩＶ－１和ＨＩＶ－２两个多肽，建立了用混合多肽为包被抗原检测ＨＩＶ－１和ＨＩＶ－２感染的间接酶联免疫吸附法。检测４６份抗ＨＩＶ－１和ＨＩＶ－２抗体阳性血清标本以及９４份对照血清标本，与ＵＢＩ试剂比较，其阳性符合率为９７．８％，阴性符合率为１００％，总符合率为９９．３％。实验结果表明，此法可用于ＨＩＶ－１和ＨＩＶ－２感染的检测。
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| 关 键 词： | 合成多肽，间接酶联免疫吸附法，人免疫缺陷病毒1型，人免疫缺陷病毒2型 |
A Study on the Detection of Antibodies to HIV Type 1 and Type 2 by Using Synthetic Peptidcs |
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| Hong Shiwen, He Hongxia, Zhu Fenglan,Lin Tao.A Study on the Detection of Antibodies to HIV Type 1 and Type 2 by Using Synthetic Peptidcs[J].中国病毒学(英文版),1995(3). |
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| Authors: | Hong Shiwen He Hongxia Zhu Fenglan Lin Tao |
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| Abstract: | wo peptides of human immunodeficiency virus(HIV)type 1 and type 2 were synthe-sized by solid-phase method. An indirect enzyme-linked immunosorbent assay(ELISA)em-ploying these peptides as an antigen adsorbent was developed to detect the infections of HIV-1 and HIV-2.A comparison of our reagent with UBI reagent in detection of 46 positive serumspecimens and 94 negative control serum specimens for HIV-1 and HIV-2 showed that theconcident rate for positive was 97.8%, for negative was 100%,and the general coincidentrate was 99.3%. The results indicated that this method can be used in detection for infectionsof HIV-1 and HIV-2. |
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| Keywords: | Synthetic peptide Indirect enzyme-linked immunosorbent assay Human immun-odeficiency virus type 1 Human immunodeficiency virus type 2 |
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