| 插入CpG序列的汉坦病毒S基因真核表达载体的构建及表达 |
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| 引用本文： | 郑兰艳,罗恩杰,李新鸣,牟玲,张小棠,鲁润铭.插入CpG序列的汉坦病毒S基因真核表达载体的构建及表达[J].Virologica Sinica,2004,19(1):73-75. |
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| 作者姓名： | 郑兰艳 罗恩杰 李新鸣 牟玲 张小棠 鲁润铭 |
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| 作者单位： | [1]中国医科大学基础医学院病原生物教研室，辽宁沈阳l1000l [2]沈阳市传染病院中心研究室，辽宁沈阳110003 |
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| 基金项目： | 沈阳市科委课题(沈科发2001-38号) |
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| 摘 要： | To improve the effect of the gene immunization against Hantaan virus, we constructed the eukaryotic expression vector pTARGET-hans(ISS) containing Hantaan Virus S gene coding region and CpG motif by cloning S gene segment with CpG motif into eukaryotic expression vector pTARGET^TM.After conformed by enzyme analysis, the recombinant expression vector pTARGET-hans(ISS) was transferred into Vero-E6 cells by electroporation and the transient expression of Hantaan virus nucleocapsid protein was detected by indirect immunofluorescence assay(IFA). In some transferred Vero-E6, the green fluorescence was showed, thus we can conclude that the eukaryotic expression vector pTARGET-hans(ISS) was successfully constructed and expressed in vitro,which will lay a foundation for further animal vaccination.
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| 关 键 词： | 汉滩病毒 真核表达载体 电穿孔 瞬例表达 肾综合征出血热 |
Construction and Expression of Eukaryotic Expression vector containing Hantaan Virus S gene and CpG motif |
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| ZHENG Lan-yan,LUO En-jie,LI Xin-ming,MOU Ling,ZHANG Xiao-tang,LU Run-ming.Construction and Expression of Eukaryotic Expression vector containing Hantaan Virus S gene and CpG motif[J].中国病毒学(英文版),2004,19(1):73-75. |
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| Authors: | ZHENG Lan-yan LUO En-jie LI Xin-ming MOU Ling ZHANG Xiao-tang LU Run-ming |
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| Institution: | ZHENG Lan-yan1,LUO En-jie1**,LI Xin-ming1,MOU Ling2,ZHANG Xiao-tang2,LU Run-ming2 |
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| Abstract: | To improve the effect of the gene immunization against Hantaan virus, we constructed the eukaryotic expression vector pTARGET-hans(ISS) containing Hantaan Virus S gene coding region and CpG motif by cloning S gene segment with CpG motif into eukaryotic expression vector pTARGETTM. After conformed by enzyme analysis, the recombinant expression vector pTARGET-hans(ISS) was transferred into Vero-E6 cells by electroporation and the transient expression of Hantaan virus nucleocapsid protein was detected by indirect immunofluorescence assay(IFA). In some transferred Vero-E6, the green fluorescence was showed, thus we can conclude that the eukaryotic expression vector pTARGET-hans(ISS) was successfully constructed and expressed in vitro, which will lay a foundation for further animal vaccination. |
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| Keywords: | Hantaan virus Eukaryotic expression vector Electroporation Transient expression |
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