| 表达LacZ基因的重组CVI988病毒的构建及特性研究 |
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| 引用本文： | 邱亚峰,葛菲菲,张雪莲,徐学清,陈溥言.表达LacZ基因的重组CVI988病毒的构建及特性研究[J].Virologica Sinica,2006,21(1):71-74. |
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| 作者姓名： | 邱亚峰 葛菲菲 张雪莲 徐学清 陈溥言 |
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| 作者单位： | 南京农业大学动物疫病诊断与免疫重点实验室,江苏南京210095 |
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| 基金项目： | 国家863高新技术发展项目（2002AA245051） |
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| 摘 要： | 马立克氏病（MD）是一种鸡的淋巴增生性肿瘤疾病，是由马立克氏病病毒血清1型（Marek＇s disease virus serotype 1，MDV1）引起的，但是，可以由致弱的或天然不致病的疫苗株进行防制。疫苗株分为三种类型：致弱的血清1型，天然不致瘤的血清2型（MDV2）和天然不致病的火鸡疱疹病毒（HVT）。
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| 关 键 词： | 重组病毒 马立克氏病毒 LacZ基因 启动子 |
| 收稿时间： | 2005-06-07 |
| 修稿时间： | 2005-09-12 |
Construction and Characterization of Recombinant Marek's Disease Virus CVI988 Expressing the E.coli LacZ Gene |
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| QIU Ya-feng, GE Fei-fei, ZhANG Xue-lian, XU Xue-qing, CHEN Pu-yan.Construction and Characterization of Recombinant Marek's Disease Virus CVI988 Expressing the E.coli LacZ Gene[J].中国病毒学(英文版),2006,21(1):71-74. |
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| Authors: | QIU Ya-feng GE Fei-fei ZhANG Xue-lian XU Xue-qing CHEN Pu-yan |
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| Institution: | Key Laboratory of Animal Disease Diagnostic and Immunology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095,China |
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| Abstract: | The LacZ gene controlled under the Marek's disease virus(MDV)glycoprotein B(gB)promoter was excised from plasmid pSK-gB-LacZ and inserted into US10 gene.Two plasmids were constructed,pUS-gB-LacZ(L)and pUS-gB-LacZ(U)),which differ with regard to the orientation of the expression cassette.Then pUS-gB-LacZ(L)was transfected into secondary Chicken embryo fibroblasts(secondary CEF)cells and super-infected with the MDV CVI988 strain.The recombinant virus(rCVILacZ)expressing the lacZ gene was isolated and purified in secondary CEF.The growth curve of rCVILacZ was similar to that of the parent virus in CEF. |
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| Keywords: | Recombinant virus Marek's disease lacZ gene Promoter |
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