| 水稻矮缩病毒昆明分离物抗血清制备及免疫捕捉PCR检测 |
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| 引用本文： | 彭潞波,李婷婷,魏春红,方琦,董家红,张丽珍,苏晓霞,丁铭,张仲凯,李毅.水稻矮缩病毒昆明分离物抗血清制备及免疫捕捉PCR检测[J].Virologica Sinica,2006,21(4):408-410. |
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| 作者姓名： | 彭潞波 李婷婷 魏春红 方琦 董家红 张丽珍 苏晓霞 丁铭 张仲凯 李毅 |
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| 作者单位： | 云南省农业科学院生物技术及种质资源研究所 云南昆明650223(彭潞波，李婷婷，方琦，董家红，张丽珍，苏晓霞，丁铭，张仲凯)，北京大学生命科学学院 北京100871(魏春红，李毅) |
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| 基金项目： | 国家重点基础研究发展规划项目(G2000016201) |
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| 摘 要： | 由水稻矮缩病毒(Rice dwarf virus,RDV)引起的水稻矮缩病害,最早由日本报道,随后在东南亚等国以及我国的福建、云南等南方稻区普遍发生,云南主要发生于中部及南部地区1]。水稻在苗期至分蘖期感病后,植株矮缩,分蘖增多,叶片浓绿,僵直,出现白斑,生长后期病稻不能抽穗结实,在暴发流行年份可以引起水稻的严重减产。RDV在分类上属于呼肠孤病毒科(Reoviridae)植物呼肠孤病毒属(Phytoreovirus)成员,病毒粒子为正十二面体球形结构,直径约为69.3nm,无刺突,无脂蛋白外膜包被,具有双层的蛋白衣壳2]。病毒基因组由12条双链RNA片段组成,其中S8编…
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| 关 键 词： | 水稻矮缩病毒 抗血清 间接ELISA 免疫捕捉PCR |
| 收稿时间： | 2005-12-26 |
| 修稿时间： | 2006-02-16 |
Antiserum Preparation against Rice Dwarf Virus Kunming Isolate and Detection by Immunocapture PCR Tests |
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| PENG Lu-bo, LI Ting-ting, WEI Chun-hong, FANG Qi, DONG Jia-hong, ZHANG Li-zhen, SU Xiao-xia, DING MingI ZHANG Zhong-Ka , LI Yi.Antiserum Preparation against Rice Dwarf Virus Kunming Isolate and Detection by Immunocapture PCR Tests[J].中国病毒学(英文版),2006,21(4):408-410. |
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| Authors: | PENG Lu-bo LI Ting-ting WEI Chun-hong FANG Qi DONG Jia-hong ZHANG Li-zhen SU Xiao-xia DING MingI ZHANG Zhong-Ka LI Yi |
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| Institution: | 1. The Biotechnology and Genetic Resources Institute, Yunnan.Academy of Agricultural Sciences, Kunming 650223, China; 2.College of Life Science, Peking University, Beijing 100871, China |
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| Abstract: | By Using the modified method to extract Rice dwarf virus virions from infected rice leaves,a concentration 3.854mg/mL of purified virus virions reached,and the Rabbits was immunized with purified virions,yielding high titer antiserum;1:20480 by the indirect enzyme-linked immunosorbent assay(I-ELISA)test.The antiserum successfully detected three rice samples from Kunming,Yuxi and Luliang by I-ELISA.Immunocapture polymerase chain reaction(IC-PCR),respectively was the utilized to show that serologic and molecular methods were successfully used for detection of Rice dwarf virus. |
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| Keywords: | Rice dwarf virus(RDV) Antiserum I-ELISA IC-PCR |
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