| 鸡传染性支气管炎病毒变异株（793／B）核蛋白基因的克隆与序列分析 |
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| 引用本文： | 孟凡磊,刁有祥,王辉,刘方娜,王妮.鸡传染性支气管炎病毒变异株（793／B）核蛋白基因的克隆与序列分析[J].Virologica Sinica,2006,21(5):468-471. |
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| 作者姓名： | 孟凡磊 刁有祥 王辉 刘方娜 王妮 |
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| 作者单位： | 山东农业大学动物科技学院，山东农业大学动物科技学院，山东农业大学动物科技学院，山东农业大学动物科技学院，山东农业大学动物科技学院 山东泰安271018，China，山东泰安271018，China，山东泰安271018，China，山东泰安271018，China，山东泰安271018，China |
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| 基金项目： | 济南市科技攻关项目(项目编号41054) |
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| 摘 要： | 根据GenBank已经发表的传染性支气管炎病毒(IBV)N全基因组序列设计引物,对IBV793/B分离毒株N基因进行克隆与序列分析。结果表明,IBV793/B的N基因由1229bp组成,与GenBank已发表的11株IBV的N基因相比较,IBV793/B的N基因共有88处点突变,在第991位发生了一个核苷酸的缺失。N基因的核苷酸同源性为86.9%～91.4%,氨基酸同源性为75.8%～77.5%。表明IBV93/B的N基因存在着较大的变异性。
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| 关 键 词： | 传染性支气管炎病毒793/B N基因 序列分析 |
| 收稿时间： | 2006-03-10 |
| 修稿时间： | 2006-04-24 |
Cloning and Sequence Analysis of the Nucleocapsid Gene of a Variant Strain (793/B) of Avian Infectious Bronchitis Virus |
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| MENG Fan-lei, DIAO You-xiang, WANG Hui, LIU Fang-na, WANG Ni.Cloning and Sequence Analysis of the Nucleocapsid Gene of a Variant Strain (793/B) of Avian Infectious Bronchitis Virus[J].中国病毒学(英文版),2006,21(5):468-471. |
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| Authors: | MENG Fan-lei DIAO You-xiang WANG Hui LIU Fang-na WANG Ni |
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| Abstract: | A pair of primers were designed and synthesized based onthe genomic sequences of the Nucleocapsid gene of IBV in Genbank. The gene encoding the nucleocapsid protein (N) was amplified by RT-PCR. Sequencing data showed that the complete gene encoding the nucleocapsid of IBV isolate (793/B) consisted of 1229 nucleotides. Sequence analysis showed that 88 point mutations were found in the N gene, and one nucleotide was deleted inform position 991. By comparison with 11 strains in GenBank, the sequence identity was 86.9%~91.4%, and the identity of the deduced amino acids was 75.8%~77.5%. The results showed that there were major variatons in the N gene of 793/B. |
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| Keywords: | 793/B strain of Infectious bronchitis virus Nucleocapsid gene Sequence analysis |
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