Two types of heat tolerance in FHM-cells. Induction by heat-shock versus elevated culturing temperature

1. 1.Increased heat tolerance in FHM-cells from Pimephales promelas (Pisces) can be induced by culturing the cells at elevated temperatures (heat resistant acclimation) as well as by heat shock (heat hardening).

2. 2.After shift of culturing temperature (CT) from 16 to 32°C both effects are detectable with different temporal patterns.

3. 3.Cellular concentrations of heat-shock proteins correlate with the hardening effect but not with heat resistance acclimation.

4. 4.Several culturing temperature specific proteins were detected. The patterns of some enzymes are also altered by culturing temperature.

5. 5.Heat resistance acclimation is not caused by selection of a thermoresistant subpopulation of cells.

6. 6.Heat hardening and heat resistance acclimation must be distinguished as different phenomena in FHM-cells.

Author Keywords: Culturing temperature specific proteins; esterase isoenzymes; FHM-cells; heat hardening; heat-shock proteins; heat resistance acclimation; thermotolerance