Combined effect of temperature and zinc on Caenorhabditis elegans wild type and daf-21 mutant strains |
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| Institution: | 1. Key Laboratory of Wetland Ecology and Environment, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China;2. National Laboratory of Protein Engineering and Plant Genetic Engineering, School of Life Sciences, Peking University, Beijing 100871, China;1. Stockholm University, Department of Applied Environmental Science (ITM), Svante Arrhenius väg 8, S-106 91 Stockholm, Sweden;2. RWTH Aachen University, ABBt – Aachen Biology and Biotechnology, Institute for Environmental Research, Department of Ecosystem Analysis, Worringerweg 1, D-520 74 Aachen, Germany;3. RWTH Aachen University, ABBt – Aachen Biology and Biotechnology, Institute for Environmental Research, Chair of Environmental Biology and Chemodynamics, Worringerweg 1, D-520 74 Aachen, Germany;1. Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária krt. 21, H-1143 Budapest, Hungary;2. Institute of Biology, Faculty of Sciences, University of Pécs, Ifjúság útja 6., H-7624 Pécs, Hungary;3. János Szentágothai Research Center, University of Pécs, Ifjúság útja 20., H-7624 Pécs, Hungary;4. Department of Veterinary Public Health, University of Bari, S.p. per Casamassima km 3, 70010 Valenzano, Bari, Italy;5. Division of Biological Standardisation, Indian Veterinary Research Institute (IVRI), Izatnagar, 243 122 Bareilly, Uttar Pradesh, India;6. Ceva-Phylaxia Veterinary Biologicals Co. Ltd., Szállás u. 5., H-1107 Budapest, Hungary;1. Department of Chemical Engineering, University of Concepción, Concepción, Chile;2. Microbiology Department, University of Concepción, Chile |
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| Abstract: | Heavy metal pollution in aquatic ecosystems is a far reaching environmental problem. The possible influences of heavy metal exposure and the potential harm to organisms when combined with other environmental stressors such as temperature have been largely unexplored. An aquatic toxicity test of Caenorhabditis elegans was performed to estimate the 24 h median lethal concentration (LC50) of different zinc concentrations at different temperatures (15 °C, 20 °C, 25 °C, and 30 °C). We also examined the time course thermotolerance on wild type (N2) and daf-21 null (JT6130) adults exposed to 6.1 mM zinc at 37 °C. Hsp90 protein expression level in response to the combined effect of temperature and zinc toxicity was also investigated by both Western blots and ELISA. Our results show that C. elegans wild type nematodes exhibit severe lethal toxicity after a 24 h exposure to zinc at higher temperatures. In addition, the expression level of Hsp90 was highly inhibited in adult worms subjected to zinc stress. This toxicity assay at different temperatures provides insight into organism response to combined effects of temperature and zinc toxicity. |
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| Keywords: | Zn Toxicology Heat shock protein 90 ELISA |
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