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The development of cysteine proteases in freesia corms during responses to chilling |
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| Authors: | Tetsuya Uchikoba Shigeko Fukumoto Michio Onjo Michiko Okubo Kazunari Arima Hiroo Yonezawa |
| Institution: | a The Kagoshima University Musium, 1-21-30, Korimoto, Kagoshima 890-0065, Japan b Ibusuki Experimental Botanical Garden, Faculty of Agriculture, Kagoshima University, 1291 Ju-cho, Ibusuki, 891-0402, Japan c Department of Health and Nutrition, Kagoshima Immaculate Heart University 2365 Amadatsu-cho, Sendai-shi, Kagoshima 895-0011, Japan d Laboratory of Biochemistry, Department of Chemistry, Faculty of Science, Kagoshima University 1-21-35, Korimoto, Kagoshima 890-0065, Japan |
| Abstract: | Freesia protease (FP)-A has been found in regular freesia corms (Kaneda et al., Biosci. Biotechnol. Biochem. 61 (1997) 1554). New corms were generated from original corms that were kept for several months at 4°C. In this study, two proteases (FP-B and FP-C) have been found to new corms kept for 6 months at 4°C, and have increased during new corms enlargement. FPs were purified from the extracts of new corms, and the Mr of those were 24k (A), 25k (B), and 24.5k (C) by SDS-PAGE, respectively. The N-terminal sequences of FPs were identical to those of papain with respect to the conservative residues of cysteine protease. The sequence of FP-A was identical with those of FP-B within 20 residues of its N-terminal. It may be possible that FP-B was produced by some post-translational modifications from FP-A during the chilling. On the other hand, N-terminal sequence of FP-C was different from those of FP-A and FP-B. It was explained that FP-C was a new protease of freesia corm. |
| Keywords: | Plant corm Cysteine protease Plant endopeptidase Freesia Freesia reflacta Storage proteins Chilling tolerance |
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