Degradation of endocrine disrupting chemicals by genetic transformants with two lignin degrading enzymes in <Emphasis Type="Italic">Phlebia tremellosa</Emphasis> |
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Authors: | Hyunwoo Kum Sungsuk Lee Sunhwa Ryu Hyoung T Choi |
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Institution: | (1) Department of Biochemistry, Molecular Microbiology Lab, Kangwon National University, Chunchon, 200-701, Korea;(2) Division of Wood Chemistry and Microbiology, Microbial Chemistry Lab, Korea Forest Research Institute, Seoul, 130-712, Korea; |
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Abstract: | A white rot fungus Phlebia tremellosa produced lignin degrading enzymes, which showed degrading activity against various recalcitrant compounds. However, manganese
peroxidase (MnP) activity, one of lignin degrading enzymes, was very low in this fungus under various culture conditions.
An expression vector that carried both the laccase and MnP genes was constructed using laccase genomic DNA of P. tremellosa and MnP cDNA from Polyporus brumalis. P. tremellosa was genetically transformed using the expression vector to obtain fungal transformants showing increased laccase and MnP
activity. Many transformants showed highly increased laccase and MnP activity at the same time in liquid medium, and three
of them were used to degrade endocrine disrupting chemicals. The transformant not only degraded bisphenol A and nonylphenol
more rapidly but also removed the estrogenic activities of the chemicals faster than the wild type strain. |
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