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Construction and characterisation of an antifungal recombinant Bacillus thuringiensis with an expanded host spectrum |
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| Authors: | Qin Liu Jong Yul Roh Yong Wang Jae Young Choi Xue Ying Tao Jae Su Kim Yeon Ho Je |
| Institution: | 1. Department of Agricultural Biotechnology, College of Agriculture and Life Science, Seoul National University, Seoul, 151-742, Republic of Korea 2. Division of Medical Entomology, Korea National Institute of Health, Chungbuk, 363-951, Republic of Korea 3. School of Life Science, Lanzhou University, 99 Dong Ganxi Road, Lanzhou, 730000, P. R. China 4. Department of Agricultural Biology, College of Agricultural Life Science, Chonbuk National University, Jeonju, 561-756, Republic of Korea 5. Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-742, Republic of Korea |
| Abstract: | A novel antifungal Bacillus thuringiensis strain 19–22, ssp. kurstaki (H3a3b3c), was characterised. This strain included cry1Aa, cry1Ab, cry1Ac, and cry1D, which have high insecticidal activities against lepidopteran larvae other than Spodoptera exigua. To expand the host spectrum, a cry1E gene whose product is active against S. exigua was introduced into the isolate. The transformant successfully expressed the Cry1E protein without any loss of its original antifungal activities. These results indicate that this recombinant strain exhibits dual activities and may be used as an integrated control agent to control plant diseases and insect pests. |
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