Preservation of capsular material of streptococcal cells by specific lectins determined by immunoelectron microscopy |
| |
| Authors: | Agnese Molinari Graziella Orefici Gianfranco Donelli Cristina Von Hunolstein Silvia Paradisi and Giuseppe Arancia |
| |
| Institution: | (1) Department of Ultrastructures, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy;(2) Department of Bacteriology and Medical Mycology, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy |
| |
| Abstract: | Summary We describe the use of lectins as specific stabilizing agents for the polysaccharide capsular components of two Gram-positive bacteria,Streptococcus agalactiae andStreptococcus bovis. Treatment of bacterial suspensions with wheatgerm agglutinin and concanavalin A allowed better morphological preservation as well as immunoelectron microscopic localization of a capsular component (lipoteichoic acid) by employing specific antibodies and the protein A-gold technique. Data obtained indicate that lectins are useful agents in preserving highly water-soluble capsular components during the electron microscopy procedures for both unembedded and embedded samples. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
