Interaction of antimicrobial cyclic lipopeptides from Bacillus subtilis influences their effect on spore germination and membrane permeability in fungal plant pathogens |
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| Institution: | 1. Institute of Pharmaceutical Microbiology, University of Bonn, Meckenheimer Allee 168, 53115 Bonn, Germany;2. Department of Pharmaceutical Biology, Pharmaceutical Institute, University of Tuebingen, Auf der Morgenstelle 8, 72076 Tuebingen, Germany;3. German Centre for Infection Research (DZIF), partner site Bonn-Cologne, Bonn, Germany;1. Département de phytologie, Université Laval, Québec, QC G1V 0A6, Canada;2. Premier Tech, 1 avenue Premier, Rivière-du-Loup, QC G5R 6C1, Canada;3. Département de médecine moléculaire, Université Laval, Québec, QC G1V 0A6, Canada;4. Centre de recherche et d''innovation sur les végétaux, Université Laval, Québec, QC G1V 0A6, Canada;1. Laboratoire de Génie Enzymatique et de Microbiologie, Université de Sfax, Ecole Nationale d’Ingénieurs de Sfax, B.P. 1173-3038 Sfax, Tunisia;2. ProBioGEM – EA1026, Polytech’Lille/IUTA, Université Lille-Nord de France, F-59655 Villeneuve d’Ascq, France;1. Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok, 10900, Thailand;2. Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, 73170, Thailand;3. Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, 12120, Thailand;4. Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand;5. Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, 10900, Thailand;1. Laboratory of Phytopathology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium;2. Charles Viollette Institute, Regional Research Laboratory for Food and Biotechnology – ProBioGEM Team, EA1026, Polytech''Lille, University Lille 1, Sciences and Technologies, Avenue Paul Langevin, F-59 655 Villeneuve d''Asq, France |
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| Abstract: | Bacillus subtilis cyclic lipopeptides are known to have various antimicrobial effects including different types of interactions with the cell membranes of plant pathogenic fungi. The various spectra of activities of the three main lipopeptide families (fengycins, iturins, and surfactins) seem to be linked to their respective mechanisms of action on the fungal biomembrane. Few studies have shown the combined effect of more than one family of lipopeptides on fungal plant pathogens. In an effort to understand the effect of producing multiple lipopeptide families, sensitivity and membrane permeability of spores from four fungal plant pathogens (Alternaria solani, Fusarium sambucinum, Rhizopus stolonifer, and Verticillium dahliae) were assayed in response to lipopeptides, both individually and as combined treatments. Results showed that inhibition of spores was highly variable depending on the tested fungus-lipopeptide treatment. Results also showed that inhibition of the spores was closely associated with SYTOX stain absorption suggesting effects of efficient treatments on membrane permeability. Combined lipopeptide treatments revealed additive, synergistic or sometimes mutual inhibition of beneficial effects. |
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| Keywords: | Fengycin Iturin Mutual inhibition Surfactin Synergistic effect |
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