Selective isolation of agents of chromoblastomycosis from insect-associated environmental sources |
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| Institution: | 1. Graduate Program in Microbiology, Parasitology and Pathology, Federal University of Paraná, Curitiba, Brazil;2. University Center Campo Real, Guarapuava, Brazil;3. Federal University of Paraná, Curitiba, Brazil;4. Graduate Program in Pharmacology, UFPR, Curitiba, Brazil;5. Medical Department, Federal University of Maranhão, São Luís, Brazil;6. Embrapa Amazônia Oriental, CPATU, Belém, Brazil;7. Westerdijk Fungal Biodiversity Institute, Utrecht, the Netherlands;8. Department of Biochemistry and Microbiology, Institute of Biological Sciences, UNESP-São Paulo State University, Rio Claro, Brazil;9. Division of Microbial Resources, CPQBA, University of Campinas, Paulínia, Brazil;10. Center of Expertise in Mycology of Radboud, University Medical Center, Canisius Wilhelmina Hospital, Nijmegen, the Netherlands;1. University of Gdańsk, Environmental Chemistry and Ecotoxicology, 80-308, Gdańsk, Poland;2. Environmental and Computational Chemistry Group, University of Cartagena, 130015, Cartagena, Colombia;3. Medicinal Plants Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650200, China;4. Adam Mickiewicz University, Department of Trace Element Analysis by Spectroscopy Method, 61-614, Poznań, Poland;5. Faculty of Science and Technology, Athabasca University, Athabasca, Alberta, T9S 3A3, Canada;1. Longping Branch, Graduate College, Hunan University, Changsha, 410125, China;2. Hunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha, 410125, China;3. College of Plant Health and Medicine, The Key Laboratory of Integrated Crop Pest Management of Shandong Province, Qingdao Agricultural University, Qingdao, 266109, China;4. Shenyang Agricultural University, Plant Protection College, Shenyang, 110866, China;1. Posgrado en Ciencias Biológicas, Universidad Nacional Autónoma de México, A.P 70-153, Ciudad de México, C.P. 04510, Mexico;2. Instituto de Biología, Universidad Nacional Autónoma de México, A.P. 70-233, Ciudad de México, C.P. 04510, Mexico;3. Programa de Investigación de Producción de Biomoléculas, Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, AP. 70-228, Ciudad de México, C.P. 04510, Mexico;1. Department of Plant Pathology, University of Florida, 2517 Fifield Hall, Gainesville, FL 32611-0680, USA;2. Department of Plant Pathology and Microbiology, University of California, Riverside, CA 92521, USA;3. Laboratory of Forest Pathology and Mycology, Graduate School of Bioresources, Mie University, Kurimamachiya 1577, Tsu, Mie 514-8507, Japan |
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| Abstract: | Chromoblastomycosis is a neglected disease characterized by cutaneous, subcutaneous or disseminated lesions. It is considered an occupational infectious disease that affects mostly rural workers exposed to contaminated soil and vegetal matter. Lesions mostly arise after a traumatic inoculation of herpotrichiellaceous fungi from the Chaetothyriales order. However, the environmental niche of the agents of the disease remains obscure. Its association with insects has been predicted in a few studies. Therefore, the present work aimed to analyze if social insects, specifically ants, bees, and termites, provide a suitable habitat for the fungi concerned. The mineral oil flotation method was used to isolate the microorganisms. Nine isolates were recovered and phylogenetic analysis identified two strains as potential agents of chromoblastomycosis, i.e., Fonsecaea pedrosoi CMRP 3076, obtained from a termite nest (n = 1) and Rhinocladiella similis CMRP 3079 from an ant exoskeleton (n = 1). In addition, we also identified Fonsecaea brasiliensis CMRP 3445 from termites (n = 1), Exophiala xenobiotica CMRP 3077 from ant exoskeleton (n = 1), Cyphellophoraceae CMRP 3103 from bees (n = 1), Cladosporium sp. CMRP 3119 from bees (n = 1), Hawksworthiomyces sp. CMRP 3102 from termites (n = 1), and Cryptendoxyla sp. from termites (n = 2). The environmental isolate of F. pedrosoi CMRP 3076 was tested in two animal models, Tenebrio molitor and Wistar rat, for its pathogenic potential with fungal retention in T. molitor tissue. In the Wistar rat, the cells resembling muriform cells were observed 30 d after inoculation. |
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| Keywords: | Black yeasts |
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